444180

MAP Kinase Inhibitor Set I

The MAP Kinase Inhibitor Set I controls the biological activity of MAP Kinase. This small molecule/inhibitor is primarily used for Phosphorylation & Dephosphorylation applications.

• 分類程式碼代碼: 12352200
• NACRES: NA.77

屬性

• 品質等級: 100
• 形狀: solid
• 製造商／商標名: Calbiochem^®
• 儲存條件: OK to freeze; protect from light
• 溶解度: DMSO: soluble
• 運輸包裝: ambient
• 儲存溫度: −20°C

描述

一般說明

Contains 5 mg of the MEK inhibitor PD 98059 (Cat. No. 513000), 1 mg each of the MAP kinase inhibitors SB 202190 (Cat. No. 559388) and SB 203580 (Cat. No. 559389), and 1 mg of the negative control, SB 202474 (Cat. No. 559387). Supplied with a data sheet.

MAP (mitogen-activated protein) kinases are ubiquitous serine/threonine kinases that are activated by a wide variety of extracellular signals. The MAP kinase cascade plays a crucial role in signal transduction from the membrane to the nucleus and appears to be highly conserved throughout evolution. In mammalian cells, this cascade is activated by both tyrosine kinase receptors and G-protein coupled receptors. MAP kinase activation, an essential step in triggering cell cycle progression, leads to changes in properties of cytoplasmic, membrane-associated and nuclear proteins. A unique feature of MAP kinases is that their full activation requires phosphorylation of both a threonine residue and a tyrosine residue which are separated by a single amino acid. This phosphorylation is brought about by the action of MAP kinase kinase (MEK), a dual specificity kinase. Once activated, MAP kinase relays signals downstream by phosphorylating various other protein kinases and regulatory proteins. Following activation, MAP kinases translocate from the cytosol into the nucleus and phosphorylate a number of transcription factors leading to changes in gene expression. Supplied with a data sheet.

生化／生理作用

Primary Target
p38 kinase

Target IC₅₀: 350 nM, 600 nM

警告

Toxicity: Standard Handling (A)

重構

Following reconstitution, aliquot and store at -20°C.

法律資訊

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全資訊

• 象形圖: GHS07
• 訊號詞: Warning
• 危險聲明: H302
• 防範說明: P264 - P270 - P301 + P312 - P501
• 危險分類: Acute Tox. 4 Oral
• 儲存類別代碼: 11 - Combustible Solids