402085

Indirubin-3′-monoxime

A potent, reversible, and ATP-compatible inhibitor of GSK-3β (glycogen synthase kinase 3β), Cdk1 (cyclin-dependent kinase1) and Cdk5 (IC₅₀ = 22 nM, 180 nM, and 100 nM, respectively).

• 同義詞: Indirubin-3′-monoxime
• 經驗公式（希爾表示法）: C₁₆H₁₁N₃O₂
• CAS號碼: 160807-49-8
• 分子量：: 277.28
• MDL號碼: MFCD02683594
• 分類程式碼代碼: 12352200
• NACRES: NA.77

屬性

• 品質等級: 100
• 化驗: ≥98% (HPLC)
• 形狀: solid
• 製造商／商標名: Calbiochem^®
• 儲存條件: OK to freeze; protect from light
• 顏色: dark red
• 溶解度: DMSO: 5 mg/mL
• 運輸包裝: ambient
• 儲存溫度: −20°C

描述

一般說明

A potent cell-permeable, reversible, and ATP-compatible inhibitor of glycogen synthase kinase-3β (IC₅₀ = 22 nM) and cyclin-dependent kinases (IC₅₀ = 180 nM for Cdk1 and 100 nM for Cdk5). Inhibits the proliferation of a large range of cells by arresting them in the G₂/M phase of the cell cycle. Inhibits Tau phosphorylation in vitro and in vivo at Alzheimer′s disease-specific sites. Also reported to inhibit the in vivo phosphorylation of DARPP-32 by Cdk5 on Thr⁷⁵.

A potent, reversible, and ATP-compatible inhibitor of GSK-3β (glycogen synthase kinase 3β), Cdk1 (cyclin-dependent kinase1) and Cdk5 (IC₅₀ = 22 nM, 180 nM, and 100 nM, respectively). Inhibits the proliferation of a large range of cells by arresting them in the G₂/M phase of the cell cycle. Inhibits Tau phosphorylation in vitro and in vivo at Alzheimer’s disease-specific sites. Also reported to inhibit the in vivo phosphorylation of DARPP-32 by Cdk5 on Thr⁷⁵.

生化／生理作用

Cell permeable: yes

Primary Target
Gsk-3β

Product competes with ATP.

Reversible: yes

Target IC₅₀: 22 nM, 180 nM, and 100 nM against GSK-3β, Cdk1, and Cdk5, respectively

包裝

Packaged under inert gas

警告

Toxicity: Standard Handling (A)

重構

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

其他說明

Bain, J., et al. 2003. Biochem. J. 371, 199.
Leclerc, S., et al. 2000. J. Biol. Chem.276, 251.
Hoessel, R., et al. 1999. Nat. Cell Biol. 1, 60.

法律資訊

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全資訊

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable