393207-M

Anti-HNE-Michael Adducts, Reduced Rabbit pAb

liquid, Calbiochem^®

• 同義詞: Anti-4-Hydroxy-2-noneal Michael Adducts, Reduced
• 分類程式碼代碼: 12352203

屬性

• 生物源: rabbit
• 品質等級: 100
• 抗體表格: serum
• 抗體產品種類: primary antibodies
• 無性繁殖: polyclonal
• 形狀: liquid
• 不包含: preservative
• 物種活性（以同源性預測）: all
• 製造商／商標名: Calbiochem^®
• 儲存條件: OK to freeze; avoid repeated freeze/thaw cycles
• 同型: IgG
• 儲存溫度: −70°C
• 目標翻譯後修改: unmodified

描述

一般說明

Anti-HNE-Michael Adducts, Reduced, rabbit polyclonal, specifically recognizes chemically reduced amino acid-(4)-HNE adducts. It is validated for use in ELISA, WB & IHC (frozen & paraffin sections).

Rabbit polyclonal antibody supplied as diluted serum. Recognizes, specifically, chemically reduced amino acid-(4)-HNE adducts.

Recognizes chemically reduced amino acid-(4)-HNE adducts. Does not recognize non-reduced adducts or reduced modifications arising from malondialdehyde (MDA) and structurally-related alkenes such as trans-2-noneal.

The CNS is enriched in polyunsaturated fatty acids and is therefore particularly vulnerable to lipid peroxidation. 4-Hydroxy-2-noneal (HNE) is one of the major lipid peroxidation products with cytotoxic and mutagenic activity. It reacts with protein residues such as histidine to generate stable Michael adducts. HNE has been shown to inhibit neurite outgrowth and disrupt microtubules and tubulin modification, suggesting it may play a role in Alzheimer′s disease.

特異性

A broad range of species

免疫原

HNE-treated KLH in which 1:1 amino acid-HNE Michael adducts were chemically reduced

應用

ELISA (1:500-1:1000)

Immunoblotting (1:1000-1:4000)

Frozen Sections (1:50-1:500)

Paraffin Sections (1:50-1:500)

警告

Toxicity: Standard Handling (A)

外觀

Serum diluted in 50% glycerol, pH 7.5.

重構

Following initial thaw, aliquot and freeze (-70°C).

其他說明

Does not bind non-reduced adducts and reduced modifications arising from malondialdehyde (MDA) or structurally-related alkenals such as trans-2-noneal. Does not cross-react with non-reduced amino acid-HNE Michael adducts. Antibody binding requires the presence of the 4-hydroxyl group. Variables associated with assay conditions will dictate the optimal working dilution.

Toyokuni, S., et al. 2000. Antioxid. Redox. Signal2, 681.
Neely, M.D., et al. 1999. J. Neurochem.72, 2323.
Uchida, K., et al. 1995. Biochem. Biophys. Res. Commun.212, 1068.
Uchida, K., et al. 1995. Arch. Biochem. Biophys.324, 241.
Uchida, K., et al. 1994. Biochemistry33, 12487.

法律資訊

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全資訊

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 1