371206

Dkk-1, His•Tag^®, Mouse, Recombinant

Dkk-1, His•Tag, Mouse, Recombinant, consists of amino acids 30-272, fused at the C-terminus to a His•Tag sequence, and expressed in NS0-dervied mouse myeloma cells.

• 分類程式碼代碼: 12352203
• NACRES: NA.41

屬性

• 重組細胞: expressed in mouse NSO cells (mouse myeloma cells)
• 品質等級: 100
• 化驗: ≥95% (SDS-PAGE)
• 形狀: lyophilized
• 製造商／商標名: Calbiochem^®
• 儲存條件: OK to freeze
• 雜質: ≤1.0 EU/μg protein Endotoxin
• 運輸包裝: ambient
• 儲存溫度: −20°C

描述

一般說明

Dkk-1, His•Tag, Mouse, Recombinant, consists of amino acids 30-272, fused at the C-terminus to a His•Tag sequence, and expressed in NS0-dervied mouse myeloma cells.

Recombinant, murine Dickkopf related protein-1 (Dkk-1), consisting of amino acids 30-272, fused at the C-terminus to a His•Tag sequence, and expressed in NS0-dervied mouse myeloma cells. Dkk-1 is a well-documented antagonist of the Wnt signaling pathway. The balance between Dkk-1 inhibition and Wnt signaling is essential for bone formation and homeostasis.

生化／生理作用

ND₅₀: 0.2-1 µg/ml as measured by the inhibition of Wnt-3a-induced alkaline phosphatase production in MC3T3-E1 mouse osteoblastic cells.

Reduction of Axis-inducing activity: 60-100% reduction (using 1 ng rmDkk-1 per embryo) in the number of secondary axes produced in the presence of 10-20 pg Xwnt-8 mRNA using early Xenopus embryos.

警告

Toxicity: Regulatory Review (Z)

外觀

Lyophilized from 0.2 µm filtered PBS, BSA.

重構

Following reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.

Reconstitute to 100 µg/ml with sterile PBS containing ≥1% human or bovine serum albumin.

其他說明

Pinzone, J.J. et al. 2009. Blood113, 517.
Li, J. et al. 2006. Bone36, 754.
Morvan, F. et al. 2006. J. Bone Miner. Res.21, 934.
Niehrs, C. 2006. Oncogene25, 7469.
Verani, R. et al. 2006. J. Neurochem.101, 242.
Cappuccio, I. et al. 2005. J. Neurosci.25, 2647.
Kemp, C. et al. 2005. Dev. Dyn.233, 1064.
Adamska, M. et al. 2004. Dev. Biol.272, 134.
Bullock, C.M. et al. 2004. Mol. Pharmacol.65, 582.
Mao, B. et al. 2002. Nature417, 664.
Mao, B. et al. 2001. Nature411, 321.
Glinka, A. et al. 1998. Nature391, 357.

法律資訊

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 2
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable