33-036

PI 3-Kinase HTRF^® Assay; 10,000 wells

The PI 3-Kinase HTRF Assay is a high-performance assay kit that provides a universal method for testing all Class I PI3-Kinases in a homogeneous format. This pack size has a screening capacity of 10,000 wells.

• 分類程式碼代碼: 12161503
• eCl@ss: 32161000
• NACRES: NA.84

屬性

• 品質等級: 100
• 物種活性: mouse, human
• 製造商／商標名: HTRF^®; Upstate^®
• 技術: activity assay: suitable (kinase)
• NCBI登錄號: NM_006218.2
• UniProt登錄號: P42336
• 檢測方法: fluorometric (HTRF)
• 運輸包裝: dry ice
• 基因資訊: human ... PIK3CA(5290)

描述

一般說明

Phosphoinositide (PI) 3-kinase (PI3K) is a heterodimeric lipid kinase containing regulatory and catalytic subunits. It catalyzes the phosphorylation at the 3-position hydroxyl group of the inositol ring of phosphatidylinositol (PtdIns). PI3K is implicated in various cellular functions such as cell growth, proliferation, motility, differentiation, survival, apoptosis, cytoskeletal rearrangement, and intracellular trafficking. It also plays a vital role in glucose uptake triggered by insulin.

應用

PI 3-Kinase HTRF^® Assay; 10,000 wells has been used for measuring the enzymatic activity of the class I phosphoinositide (PI) 3-kinase isoforms by a time-resolved fluorescence resonance energy transfer (TR-FRET) assay.

生化／生理作用

Target Sub-Family: PI3K/PI4K

包裝

Kit capacity: 10,000 wells

成分

4X Reaction Buffer

PIP2 (Substrate; 1mM stock)

Stop Reagent A

Stop Reagent B

DM A Detection Reagent

DM B Detection Reagent

DM C Detection Reagent

儲存和穩定性

12 months at -70°C

法律資訊

HTRF is a registered trademark of Cis Bio International

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.