264150

Caspase-3 Substrate IV, Fluorogenic

Fluorogenic caspase-3 substrate.

• 同義詞: Caspase-3 Substrate IV, Fluorogenic, Z-DEVD-AFC
• 經驗公式（希爾表示法）: C₃₆H₃₈F₃N₅O₁₄
• CAS號碼: 1177935-21-5
• 分子量：: 821.71
• 分類程式碼代碼: 12352202
• NACRES: NA.77

屬性

• 品質等級: 100
• 化驗: ≥95% (HPLC)
• 形狀: solid
• 製造商／商標名: Calbiochem^®
• 儲存條件: OK to freeze
• 顏色: white to off-white
• 溶解度: DMSO: 10 mg/mL
• 螢光: λ_ex ~400 nm; λ_em ~505 nm
• 運輸包裝: ambient
• 儲存溫度: −20°C

描述

一般說明

Fluorogenic CPP-32/apopain substrate. Also acts as a substrate for granzyme B-processed ICE-LAP3. Reaction can be monitored by a blue to green shift in fluorescence upon cleavage of the AFC fluorophore. Excitation max: ~400 nm; emission max: ~505 nm.

Fluorogenic caspase-3 substrate. Also acts as a substrate for granzyme B-processed ICE-LAP3. Reaction can be monitored by a blue to green shift in fluorescence upon cleavage of the AFC fluorophore. Also a substrate for caspase-6, caspase-7, caspase-8, and caspase-10.

生化／生理作用

Cell permeable: no

Primary Target
Fluorogenic caspase-3 substrate.

Product does not compete with ATP.

Reversible: no

警告

Toxicity: Standard Handling (A)

序列

Z-Asp-Glu-Val-Asp-AFC

重構

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for 3 months at -20°C.

其他說明

Keramaris, E., et al. 2000. Mol. Cell Neurosci. 15, 368.
Thornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312.
Kluck, R.M., et al. 1997. Science275, 1132.
Talanian, J., et al. 1997. J. Biol. Chem.272, 9677.
Chinnaiyan, A.M., et al. 1996. Curr. Biol.6, 897.
Nicholson, D.W., et al. 1995. Nature376, 37.

法律資訊

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全資訊

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable