251520-M

DAF-FM DA

A cell-permeable, photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~3 nM.

• 同義詞: DAF-FM DA, 3-Amino, 4-aminomethyl-2ʹ,7ʹ-difluorofluorescein Diacetate
• 經驗公式（希爾表示法）: C₂₅H₁₈F₂N₂O₇
• CAS號碼: 254109-22-3
• 分子量：: 496.42
• MDL號碼: MFCD16872050
• 分類程式碼代碼: 12352204
• NACRES: NA.25

屬性

• 品質等級: 100
• 化驗: ≥98% (HPLC)
• 形狀: solution
• 製造商／商標名: Calbiochem^®
• 儲存條件: OK to freeze; protect from light
• 顏色: pale yellow
• 螢光: λ_ex ~500 nm; λ_em ~515 nm
• 儲存溫度: −20°C
• InChI: 1S/C25H18F2N2O7/c1-10(30)33-20-8-18-13(6-15(20)26)25(12-4-5-17(29-3)23(28)22(12)24(32)36-25)14-7-16(27)21(34-11(2)31)9-19(14)35-18/h4-9,29H,28H2,1-3H3
• InChI 密鑰: BEVHTVRRVVEMEF-UHFFFAOYSA-N

描述

一般說明

A cell-permeable, photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~3 nM. Intracellularly releases DAF-FM (Cat. No. 251515) by the action of esterases. Reacts with NO, in the presence of O₂, resulting in a triazolo-fluorescein analog (DAF-FM T) that exhibits about 160-fold greater fluorescence quantum efficiency. The fluorescence is reported to be stable above pH 5.8.

A cell-permeable, photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~3 nM. Releases DAF-FM (Cat. No. 251515) by the action of intracellular esterases. Reacts with NO, in the presence of oxygen, resulting in the formation of a triazolo-fluorescein analog (DAF-FM T) that exhibits about 160-fold greater fluorescence quantum efficiency. The fluorescence intensity is reported to be stable above pH 5.8. Excitation max.: ~500 nm; emission max.: ~515 nm.

生化／生理作用

Primary Target
Nitric oxide (NO) fluorescent indicator

警告

Toxicity: Irritant (B)

外觀

A 5 mM solution in DMSO.

重構

Following initial thaw, aliquot and freeze at (-20°C). Use immediately after dilution; discard any unused portion.

其他說明

Kojima, H., et al. 2001. J. Neurochem.76, 1404.
Park. J.M., et al. 2001. Br. J. Pharmacol.132, 1876.
Itoh, Y., et al. 2000. Anal. Biochem.287, 203.
Kojima, H., et al. 1999. Angew Chem. Int. Ed.38, 3209.

法律資訊

Manufactured by Daiichi Pure Chemicals Co., Ltd

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全資訊

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 2
• 閃點（°F）: 188.6 °F - closed cup - (Dimethylsulfoxide)
• 閃點（°C）: 87 °C - closed cup - (Dimethylsulfoxide)