227036

Cholera Toxin

from Vibrio cholerae, ≥95% (SDS-PAGE), solid, A-B type toxin, Calbiochem

• 同義詞: Cholera Toxin, Vibrio cholerae, Type Inaba 569B, Azide Free
• CAS號碼: 9012-63-9
• MDL號碼: MFCD00130776
• 分類程式碼代碼: 12352202
• NACRES: NA.51

屬性

• product name: Cholera Toxin, Vibrio cholerae, Type Inaba 569B, Azide Free, Cholera toxin Inaba 569B, CAS 9012-63-9, has a single A subunit surrounded by five polypeptides chains of the B subunits. The A subunit catalyzes the ADP-ribosylation of the α-subunit of G-proteins.
• 品質等級: 100
• 化驗: ≥95% (SDS-PAGE)
• 形狀: solid
• 製造商／商標名: Calbiochem^®
• 儲存條件: do not freeze
• 運輸包裝: ambient
• 儲存溫度: 2-8°C

描述

一般說明

Useful for studies involving adenylate cyclase, AMP, and related membrane-transport phenomena. Consists of a single A subunit surrounded by five polypeptide chains of the B subunits. The A subunit catalyzes the ADP-ribosylation of the α-subunit of G-proteins, reducing intrinsic GTPase activity and activating the α-subunit. The B subunits are responsible for the attachment of the native toxin on the cell surface.

Useful for studies involving adenylate cyclase, AMP, and related membrane-transport phenomena. Consists of a single A subunit surrounded by five polypeptide chains of the B subunits. The A subunit catalyzes the ADP-ribosylation of the α-subunit of G-proteins, reducing intrinsic GTPase activity and activating the α-subunit. The B subunits are responsible for the attachment of the native toxin on the cell surface..

Useful for tissue culture applications.

生化／生理作用

Cell permeable: no

Primary Target
α-subunit of G-proteins

Product does not compete with ATP.

Reversible: no

包裝

Please refer to vial label for lot-specific concentration.

yes

警告

Toxicity: Highly Toxic (H)

外觀

Lyophilized from 200 mM NaCl, 50 mM Tris, 1 mM EDTA, pH 7.5.

重構

Following reconstitution, store in the refrigerator (4°C). Aqueous stock solutions are stable for up to 6 months at 4°C under sterile conditions.

Please refer to the vial label for lot-specific reconstitution. Note: cholera toxin tends to breakdown into its A and B subunits when the solution is frozen or with vigorous shaking during reconstitution, resulting in damage to the toxin protein and concomitant loss of biological activity. Swirl bottles gently during reconstitution. Avoid vigorous pipetting of solutions that would lead to foaming. Heating of cholera toxin in excess of 40-50°C results in aggregation and subsequent breakdown into its A and B subunits.

分析報告

Binding activity is assessed by hemagglutination assay using trisialoganglioside fixed sheep red blood cells.

single band by disc gel electrophoresis

其他說明

Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.

Gilman, A.G. 1987. Annu. Rev.Biochem.56, 615.

法律資訊

Not available for sale outside of the United States.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全資訊

• 象形圖: GHS06
• 訊號詞: Danger
• 危險聲明: H300 + H310,H315
• 防範說明: P262 - P264 - P270 - P280 - P301 + P310 - P302 + P352 + P310
• 危險分類: Acute Tox. 1 Dermal - Acute Tox. 1 Oral - Skin Irrit. 2
• 儲存類別代碼: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• 水污染物質分類（WGK）: WGK 3