194434

ABTS^™ Chromophore

• 同義詞: ABTS^™ Chromophore
• 經驗公式（希爾表示法）: C₁₈H₁₈N₄O₆S₄
• 分子量：: 514.62
• 分類程式碼代碼: 41116133
• NACRES: NA.41

屬性

• 形狀: solution
• 品質等級: 100
• 包含: ≤0.1% sodium azide as preservative
• 製造商／商標名: Calbiochem^®
• 儲存條件: OK to freeze
• 顏色: clearvery pale yellow
• 運輸包裝: ambient
• 儲存溫度: 2-8°C

描述

一般說明

Peroxidase substrate that is soluble, safe, convenient and has good overall performance in ELISA. ABTS^™ Chromophore develops intense blue-green color that can be measured at 405 nm to 410 nm. May be used for both kinetic and endpoint reactions.

Single component peroxidase substrate which is soluble, safe, convenient and has good overall performance for ELISA. In the presence of horseradish peroxidase (HRP) and hydrogen peroxide, ABTS is oxidized to an intense blue-green, water-soluble product. This product is a radical cation having an absorption maximum at 416 nm. Typically, product formation is measured spectrophotometrically at 405 nm, allowing a quantitative analysis of HRP-based systems. The color formation as a function of time may be monitored, or the reaction may be stopped by the addition of acid; since the acid does not alter the absorbance at 405 nm, one may perform both kinetic and endpoint measurements at the same wavelength.

警告

Toxicity: Irritant (B)

外觀

Contains optimal concentrations of ABTS and H₂O₂ in a citrate buffer (pH 4.0) with proprietary stabilizers.

重構

ABTS^™ solution should be stored at 4°C, but must be warmed to room temperature before use. The solution should be protected from exposure to direct sunlight, and should be discarded if it becomes bright yellow or turbid.

其他說明

Rodgriquez-Lopez, J.N., et al. 2000. Biochemistry39, 13201.

法律資訊

ABTS is a trademark of Boehringer Mannheim Corp.

ABTS is a trademark of Roche

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 2
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable