16-454

Anti-Phosphotyrosine Antibody, clone PY20, HRP Conjugate

clone PY20, Upstate^®, from mouse

• 同義詞: PY20, anti-phosphotyrosine antibody
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: mouse
• 品質等級: 100
• 共軛: peroxidase conjugate
• 抗體表格: purified immunoglobulin
• 抗體產品種類: primary antibodies
• 無性繁殖: PY20, monoclonal
• 物種活性（以同源性預測）: all
• 製造商／商標名: Upstate^®
• 技術: ELISA: suitable; western blot: suitable
• 同型: IgG2b
• 運輸包裝: wet ice
• 目標翻譯後修改: unmodified
• 基因資訊: human ... PID1(55022)

描述

一般說明

Protein tyrosine phosphorylation was first described in 1979. Large numbers of tyrosine kinases have been described since, and many of the signaling pathways initiated by tyrosine phosphorylation have been delineated. Availability of a top-quality anti-phosphotyrosine antibody has allowed the study of tyrosine kinase activity by simple blotting procedures, and without routine need of large quantities of radioactivity. The 4G10 monoclonal anti-phosphotyrosine antibody (Cat. # 05-321) has been widely recognized as a highly specific and sensitive. This antibody is suitable for all applications involving protein tyrosine phosphorylation. The slightly less sensitive, but still highly regarded clone PY20 can be used as an alternative to the 4G10 product line.

特異性

Recognizes proteins containing phosphotyrosine residues, regardless of origin.

免疫原

Epitope: phosphotyrosine

Phosphotyrosine conjugated to carrier protein

應用

Anti-Phosphotyrosine Antibody, clone PY20, HRP Conjugate is a Mouse Monoclonal Antibody for the detection of Phosphotyrosine & has been validated in WB, ELISA.

Research Category
Signaling

Research Sub Category
General Post-translation Modification

品質

Routinely evaluated by western blot on RIPA lysates from insulin-treated human HEK293 cells.

標靶描述

Varies

外觀

Protein A purified

100 μL of protein A purified mouse IgG2b in PBS containing 0.05% Kathon^® and HRP stabilizing compounds. Sufficient for a minimum of 10 immunoblots, if used at the optimum concentration of 1:2000- 1:4000 in 10 mL blocking buffer. Liquid.

儲存和穩定性

6 months at 2-8°C from date of shipment.
NOTE: DO NOT FREEZE. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

法律資訊

Kathon is a registered trademark of Rohm and Haas Co.

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 象形圖: GHS07,GHS09
• 訊號詞: Warning
• 危險聲明: H315,H317,H319,H411
• 防範說明: P261 - P264 - P273 - P280 - P302 + P352 - P305 + P351 + P338
• 危險分類: Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1
• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable