推薦產品
生物源
mouse
品質等級
共軛
biotin conjugate
抗體表格
purified antibody
抗體產品種類
primary antibodies
無性繁殖
4G10®, monoclonal
物種活性（以同源性預測）
all
製造商／商標名
Upstate®
技術
western blot: suitable
同型
IgG2bκ
運輸包裝
wet ice
目標翻譯後修改
unmodified
基因資訊
human ... PID1(55022)
一般說明
The development of the anti-phosphotyrosine, clone 4G10 in 1989 was a monumental discovery for researchers. 4G10 was the first and best single monoclonal antibody for the detection tyrosine phosphorylation. 4G10 is well known for its sensitivity and its ability to detect multiple tyrosine phosphorylations on numerous substrates. It has been validated by thousands of scientific and medical researchers in virtually every application and tyrosine target over the past 2 decades. Now Millipore has made the best even better with 4G10 Platinum. We pooled 4G10 with the next most highly regarded anti-phosphotyrosine, clone PY20, to make 4G10 Platinum. PY20 itself is a very poor substitute for 4G10, but its additive effect allow for a greater level of detection on more substrates that even 4G10 alone is capable.
免疫原
The immunogen for 4G10 was phospho-tyramine coupled to KLH, while the immunogen for PY20 was phosphotyrosine conjugated to a carrier protein.
應用
4G10 Platinum, Anti-Phosphotyrosine, Biotin Conjugate detects levels of Phosphotyrosine proteins & has been published & validated for use in IP.
品質
Western Blot Analysis:
A dilution of 1:1000-2000 of this antibody detected tyrosine-phosphorylated proteins in a modified RIPA lysate from human HEK293 cells treated with 100 nM insulin.
A dilution of 1:1000-2000 of this antibody detected tyrosine-phosphorylated proteins in a modified RIPA lysate from human HEK293 cells treated with 100 nM insulin.
標靶描述
Varies
外觀
100 μL of a proprietary mixture of biotin conjugated, protein G purified mouse monoclonals 4G10 (IgG2bκ) and PY20 (IgG2b) in PBS, pH 7.4 with 0.05% sodium azide.
儲存和穩定性
Stable for 6 months at 2-8°C from date of shipment. For maximum recovery of product, centrifuge the vial prior to removing the cap.
NOTE: DO NOT FREEZE.
NOTE: DO NOT FREEZE.
法律資訊
4G10 is a registered trademark of Upstate Group, Inc.
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 2
閃點（°F）
Not applicable
閃點（°C）
Not applicable
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。
Ren Liu et al.
Blood, 116(2), 297-305 (2010-05-06)
Axl is an oncogenic receptor tyrosine kinase that plays multiple roles in tumorigenesis and metastasis of many cancers. This study is the first to demonstrate that Axl is induced in Kaposi sarcoma and Kaposi sarcoma herpesvirus (KSHV) transformed endothelial cells.
Shalini Chaturvedi et al.
International journal of biological sciences, 9(10), 1099-1107 (2013-12-18)
A novel assay was developed to measure ratio of p-FMS (phospho FMS) to FMS using the Meso Scale Discovery(®) (MSD) technology and compared to the routinely used, IP-Western based approach. The existing IP-Western assay used lysed PBMCs (Peripheral Blood Mononuclear
Philippe Foubert et al.
The Journal of clinical investigation, 117(6), 1527-1537 (2007-05-19)
Endothelial progenitor cell (EPC) transplantation has beneficial effects for therapeutic neovascularization; however, only a small proportion of injected cells home to the lesion and incorporate into the neocapillaries. Consequently, this type of cell therapy requires substantial improvement to be of
The carboxyl terminus controls ligand-dependent activation of VEGFR-2 and its signaling
Meyer, R. D., et al
The Journal of Biological Chemistry, 279, 735-742 (2004)
Keigo Nishida et al.
The Journal of cell biology, 170(1), 115-126 (2005-07-07)
The aggregation of high affinity IgE receptors (Fcepsilon receptor I [FcepsilonRI]) on mast cells is potent stimulus for the release of inflammatory and allergic mediators from cytoplasmic granules. However, the molecular mechanism of degranulation has not yet been established. It
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