16-318

Anti-Akt/PKB Antibody, PH Domain, clone SKB1, Magnetic Bead Conjugate

clone SKB1, from mouse

• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: mouse
• 品質等級: 100
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: SKB1, monoclonal
• 物種活性: mouse, rat, human
• 技術: immunoprecipitation (IP): suitable
• 同型: IgG
• 運輸包裝: wet ice
• 目標翻譯後修改: unmodified
• 基因資訊: mouse ... Akt1(11651)

描述

一般說明

Akt (protein kinase B), a serine/threonine kinase, has emerged as a critical enzyme in signal transduction pathways involved in cell proliferation, apoptosis, angiogenesis, and diabetes. In mammals three isoforms of Akt (α, β, γ or Akt 1, 2, 3) are reported that exhibit a high degree of homology, but differ slightly in the localization of their regulatory phosphorylation sites. Akt α is the predominant isoform in most tissues, whereas the highest expression of Akt β is observed in the insulin-responsive tissues, and Akt γ is abundant in brain tissue. Each Akt isoform is composed of three functionally distinct regions: an N-terminal pleckstrin homology (PH) domain that provides a lipid-binding module to direct Akt to PIP2 and PIP3, a central catalytic domain, and a C-terminal hydrophobic motif.

特異性

Recognizes Akt1/PKBα.

免疫原

GST-fusion protein corresponding to residues 1-149 of human Akt1

應用

Detect Akt/PKB using this Anti-Akt/PKB Antibody, PH Domain, clone SKB1, Magnetic Bead Conjugate validated for use in IP.

Research Category
Signaling

Research Sub Category
PI3K, Akt, & mTOR Signaling

品質

Evaluated by immunoprecipitation on 250 μg of NIH3T3 whole cell lysate stimulated with 50 ng/mL PDGF (5 minutes).

標靶描述

~ 60 kDa observed

外觀

Protein G Chromatography

20% slurry; 1X PBS, 0.05% sodium azide.
Sufficient for 10 IPs.

Format: Purified

儲存和穩定性

Stable for 1 year at 2-8ºC from date of receipt.

分析報告

Control
NIH3T3 whole cell lysate stimulated with 50 ng/mL PDGF (5 minutes)

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 2