推薦產品
生物源
mouse
品質等級
共軛
peroxidase conjugate
抗體產品種類
primary antibodies
無性繁殖
4G10®, monoclonal
PY20, monoclonal
物種活性（以同源性預測）
all
製造商／商標名
Upstate®
技術
ELISA: suitable
western blot: suitable
同型
IgG2b
運輸包裝
wet ice
目標翻譯後修改
phosphorylation (pTyr)
基因資訊
human ... PID1(55022)
一般說明
1989年抗磷酸酪氨酸克隆4G10的开发对研究人员来说是一个巨大的发现。4G10是第一个检测酪氨酸磷酸化的单克隆抗体，是检测酪氨酸磷酸化的最佳单克隆抗体。4G10以其灵敏度和检测多种底物上多种酪氨酸磷酸化的能力而闻名。在过去的20年中，它已经在几乎每个应用和酪氨酸靶标中得到了数千名科学和医学研究人员的验证。为了改进一百种尝试过但没有人成功的东西，我们将4G10 与下一个最受推崇的抗磷酸酪氨酸克隆PY20合并在一起，制成4G10 铂。PY20本身是4G10的非常差的替代品，但是它的累加效应可在更多的底物上进行更高水平的检测，甚至可以单独使用4G10 。
應用
使用抗磷酸酪氨酸抗体，4G10铂，HRP偶联物（小鼠单克隆抗体）检测磷酸酪氨酸，该抗体已在WB，ELISA中显示有效。
品質
通过蛋白质印迹法进行了常规评估。
標靶描述
变化
外觀
100 μL经蛋白G纯化的小鼠单克隆4G10（IgG2bƙ）HRP偶联物和经蛋白A纯化的小鼠单克隆PY20，HRP偶联物（IgG2b）的专有混合物，溶于含有0.05% Kathon和HRP稳定化合物的PBS中。如果在10 mL封闭缓冲液中以1:2000 – 1:4000的最佳浓度使用，则足以进行最少10次免疫印迹。 液体。
法律資訊
4G10 is a registered trademark of Upstate Group, Inc.
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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危險分類
Aquatic Chronic 2 - Skin Sens. 1
水污染物質分類（WGK）
WGK 3
閃點（°F）
does not flash
閃點（°C）
does not flash
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。
Ondřej Ballek et al.
Immunology letters, 142(1-2), 64-74 (2012-01-28)
Lck is the principal signal-generating tyrosine kinase of the T cell activation mechanism. We have previously demonstrated that induced Lck activation outside of lipid rafts (LR) results in the rapid translocation of a fraction of Lck to LR. While this
Irmgard Hofmann et al.
The Journal of pharmacology and experimental therapeutics (2022-10-15)
VEGF and angiopoietin-2 (ANG2) have complementary roles in angiogenesis and promote an immunosuppressive tumor microenvironment. It is anticipated that combination of VEGF and ANG2 blockade could provide superior activity to blockade of either pathway alone, and that addition of VEGF/ANG2
Wei Tian et al.
BMC biochemistry, 13, 22-22 (2012-10-25)
Phosphatase of regenerating liver-3 (PRL-3 or PTP4A3) has been implicated in controlling cancer cell proliferation, motility, metastasis, and angiogenesis. Deregulated expression of PRL-3 is highly correlated with cancer progression and predicts poor survival. Although PRL-3 was categorized as a tyrosine
Pierluigi Scalia et al.
Oncogene, 38(31), 5987-6001 (2019-07-05)
Malignant mesothelioma is a deadly disease with limited therapeutic options. EphB4 is an oncogenic tyrosine kinase receptor expressed in malignant mesothelioma as well as in a variety of cancers. It is involved in tumor microenvironment mediating angiogenesis and invasive cellular
Tariq S Adwan et al.
The Journal of biological chemistry, 286(41), 35716-35724 (2011-08-26)
PKCδ translocates into the nucleus in response to apoptotic agents and functions as a potent cell death signal. Cytoplasmic retention of PKCδ and its transport into the nucleus are essential for cell homeostasis, but how these processes are regulated is
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