16-222

Anti-Phospho-Histone H3 (Ser10) Antibody, clone 3H10, FITC Conjugate

clone 3H10, Upstate^®, from mouse

• 同義詞: H3 histone, family 3A, H3S10P, Histone H3 (phospho S10), H3 histone, family 3A, H3 histone, family 3B, H3 histone, family 3B (H3.3B)
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: mouse
• 品質等級: 100
• 共軛: FITC conjugate
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: 3H10, monoclonal
• 物種活性: human
• 製造商／商標名: Upstate^®
• 技術: immunocytochemistry: suitable; immunofluorescence: suitable; western blot: suitable
• 同型: IgG
• NCBI登錄號: NP_003484
• UniProt登錄號: Q16695
• 運輸包裝: wet ice
• 目標翻譯後修改: phosphorylation (pSer10)
• 基因資訊: human ... HIST1H3F(8968)

描述

一般說明

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

特異性

Broad species cross-reactivity is expected.

Recognizes histone H3 phosphorylated at Ser10, MW 17 kDa.

免疫原

A proprietary immunogen based on a peptide sequence containing phospho-serine corresponding to residue 10 of human histone H3. Clone 3H10.

Epitope: Ser10

應用

Anti-Phospho-Histone H3 (Ser10) Antibody, clone 3H10, FITC Conjugate is a Mouse Monoclonal for detection of Histone H3 phosphorylated at serine 10. This mAb, also known as H3S10p,is labeled with Fluorescein isothiocyanate (FITC), published in peer reviewed journals & validated in WB, ICC & IF.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histones

Western Blot Analysis: 0.5-5 µg/mL of this lot detected phosphorylated histone H3 in acid extracted proteins from mitotic HeLa cells (Catalog # 17-306) treated with colcemid.

品質

Immunocytochemistry: Mitotic HeLa cells showed positive chromosome staining with 4μg/mL of this antibody.

標靶描述

17 kDa

外觀

Protein G Purified

Purified mouse monoclonal IgG in buffer containing PBS with 0.05% sodium azide, pH7.1.

儲存和穩定性

Stable for 1 year at from date of receipt.

分析報告

Control
Mitotic HeLa cells (IF).

法律資訊

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 2