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1.50443

Supelco

SeQuant ZIC®-HILIC 100Å (3.5 µm) HPLC Columns

L × I.D. 250 mm x 2.1 mm HPLC Column, PEEK-lined stainless steel

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About This Item

分類程式碼代碼:
41115709

product name

SeQuant ZIC-HILIC 3.5µm,100Å 250 x 2.1 mm, PEEK-lined stainless steel column, suitable for HPLC

材料

PEEK-lined stainless steel column

品質等級

agency

suitable for USP L114

產品線

SeQuant

特點

endcapped: no

參數

550 bar pressure
70 °C max. temp.

技術

HPLC: suitable
LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable
mass spectrometry (MS): suitable (High)

柱長度 × 內徑

25 cm × 2.1 mm

基質

fully porous particle

基質活性組

ZIC-HILIC bonding phase

粒徑

3.5 μm

孔徑

100 Å pore size

工作pH值

2-7.5

分離技術

hydrophilic interaction (HILIC)

儲存溫度

no temp limit

一般說明

SeQuant ZIC®-HILIC Columns are designed to retain and separate all types of polar and hydrophilic compounds and for robust chromatography with high selectivity and reproducibility.
The silica-based ZIC®-HILIC sorbent has a bonded stationary phase consisting of a highly polar, permanent zwitterion. Separation selectivity is favored by the 1:1 zwitterion charge balance, which makes the ZIC®-HILIC Column overall neutral, with weak, but important, ionic interactions. Tuning of the selectivity on the ZIC®-HILIC Column during method development is facilitated by the pH-inpendent permanent zwitterion, ensuring that only the analytes (and not the column) are affected during eluent optimization.
The Pore size of 100 Å makes this column perfectly suitable for the separation of small polar molecules under HILIC conditions.
The column dimension of 250 mm length x 2.1 mm ID is the optimal choice for the separation of complex samples using a UHPLC or optimized HPLC Instrument providing low dwell volumes.
Thanks to their favorable retention and robust performance, SeQuant HILIC columns have become extremely popular in numerous laboratories around the world. For example, US FDA (United States Food and Drug Administration) has recommended SeQuant ZIC®-HILIC for analysis of melamine and related substances.
SeQuant HILIC columns excel in stability and low bleed. These features make them particularly suitable for LC-MS applications where high background can lead to signal suppression and interference with quantitative measurements while also increasing instrument wear. Their high stability makes these high-performance HILIC columns also attractive for traditional HPLC applications.
Learn more about the Supelco HPLC Column portfolio and Method development:
Brochure: HPLC and UHPLC Column Selection Guide
Brochure: A Practical Guide to HPLC - Method Development Guide

分析報告

Theoretical Plates (N/m) (Cytosine): ≥ 90000
Symmetry (Cytosine): 0.95 - 1.40

法律資訊

SeQuant is a trademark of Merck KGaA, Darmstadt, Germany
ZIC is a registered trademark of Merck KGaA, Darmstadt, Germany
ZIC-HILIC is a trademark of Merck KGaA, Darmstadt, Germany

分析證明 (COA)

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