14-763-M

Tie2 (R849W) Protein, active, 250 µg

• 分類程式碼代碼: 12352202
• eCl@ss: 32160405
• NACRES: NA.32

屬性

• 重組細胞: expressed in baculovirus infected Sf21 cells
• 品質等級: 300
• 描述: protein target Tie2
• 純化經由: (Ni²⁺/NTA-agarose)
• 技術: activity assay: suitable
• UniProt登錄號: Q02858
• 基因資訊: human ... TEK(7010)

描述

一般說明

N-terminal 6His-tagged recombinant human Tie2 residues 771-end containing the R849W substitution.

The R849W mutation has been reported in dominantly inherited venous malformations. Expression studies in insect and mammalian cells demonstrates that Tie2 R849W has constitutively elevated tyrosine kinase activity compared with wild type. (Morris P.N., et al., Biochem.Biophys.Res.Commun.(2006);346:335-338 and Vikkula M, et al., Cell(1996);87:1181-1190).

包裝

Also available in 10 μg size (cat#14-763) and in bulk (cat# 14-763-K).

品質

routinely evaluated by phosphorylation of Poly (Glu4-Tyr) (4:1)

物理性質

42kDa

外觀

50mM Tris/HCl pH8.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij 35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.

儲存和穩定性

Store -70°C. For maximum recovery of product, centrifuge original vial prior to removing the cap. Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70°C.

分析報告

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.