14-429-M

MEK1 Protein, active, 10 µg

Active, N-terminal GST & C-terminal His6-tagged, recombinant full-length human MEK1, for use in Kinase Assays.

• 同義詞: CFC3, MAP2K1, MAPKK1, MEL, MKK1, PRKMK1, mitogen-activated protein kinase kinase 1
• 分類程式碼代碼: 12352202
• eCl@ss: 32160405
• NACRES: NA.41

屬性

• 生物源: human
• 品質等級: 100
• 重組細胞: expressed in E. coli
• 產品線: Upstate^®
• 形狀: liquid
• 比活性: (For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.)
• 儲存期限: 1 yr
• 分子量: M_w 71 kDa
• 純化經由: (Purified using glutathione agarose followed by Ni2+/NTA agarose, activated using c-Raf and the re-purified using Ni2+/NTA agarose)
• 物種活性: human
• 製造商／商標名: Upstate^®
• 技術: activity assay: suitable (kinase)
• 溶解度: soluble
• 登錄號: (FUNCTION: SwissProt: Q02750 # Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates ERK1 and ERK2 MAP kinases. ); NM_002755.2
• NCBI登錄號: NM_002755.2
• UniProt登錄號: Q02750
• 儲存溫度: −70°C
• 基因資訊: human ... MAP2K1(5604)

描述

一般說明

Research area: Cell Signaling Protein Target: MEK1 Target Sub-Family: STE

N-terminal GST and Cterminal His6-tagged, recombinant full-length human MEK1

生化／生理作用

Mitogen-activated protein kinase kinase 1 (MEK1) is a hydrophilic non-receptor protein kinase that shows ubiquitous expression across different cell types. It serves as a critical component of the RAS-RAF-MEK-ERK signal transduction cascade, also known as the extracellular signal-regulated kinase (ERK)/mitogen-activated protein kinase (MAPK) cascade. This signaling pathway plays a crucial role in regulating various biological processes, including apoptosis, cell cycle progression, cell migration, differentiation, metabolism, and proliferation. Additionally, MEK1 protein contributes to the development of the extra-embryonic ectoderm during placentogenesis. Somatic mutations in the MEK1 gene have been observed in individuals with melanoma, a form of skin cancer.

包裝

Also available in 10 μg size and in bulk

Also available in 250μg size as catalog # 14-429M - Call for pricing and availability.

品質

routinely evaluated by phosphorylation of MBP

標靶描述

MEK1

外觀

Purified using glutathione agarose followed by Ni2+/NTA agarose, activated using c-Raf and the re-purified using Ni2+/NTA agarose

儲存和穩定性

1 year at -70°C

其他說明

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

法律資訊

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 象形圖: GHS07
• 訊號詞: Warning
• 危險聲明: H317
• 防範說明: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• 危險分類: Skin Sens. 1
• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°C）: Not applicable