1.25137

RoboColumn Fractogel DEAE (M) 0.6 ml

RoboColumn Fractogel^® TMAE Hicap (M), volume 0.6 mL, prepacked column

• 同義詞: Fractogel^® EMD TMAE Hicap (M)
• 分類程式碼代碼: 41115711

屬性

• ligand: (Trimethylammoniumethyl)
• 品質等級: 100
• 材料: polypropylene housing
• 產品線: Fractogel^®
• 形狀: prepacked column
• 參數: ≤8 bar pressure; ~180 cm/hr max. flow rate
• 柱內徑 × 長度: 5 mm × 30 mm
• 容積: 0.6 mL
• 基質活性組: Methacrylate
• 平均粒徑: 40-90 μm
• 容量: ~180 mg, BSA binding capacity
• 應用: mAb; recombinant protein; vaccine development
• 分離技術: anion exchange
• 儲存溫度: 15-25°C

描述

一般說明

5 x 30 mm

特點和優勢

RoboColumn Fractogel^® TMAE Hicap (M), 0.6 ml enables users to:

• Efficiently capture target protein and remove viruses, DNA, and endotoxins with its tentacle technology resulting in better production yields and high throughput
• Reduce operating costs and resin replacement with enduring durability - allowing for multiple cycles of column regeneration and sanitization
• Compatibility with 2.5 % (v/v) aqueous benzyl alcohol containing 150 mM NaCl storage solution

Due to the titration behavior of Fractogel^® EMD TMAE Hicap (M) the ion exchange capacity can be used from pH 2 to 12. The separation of proteins is based on reversible electrostatic interactions between the negatively charged regions of the proteins′ surface and the support. Proteins are retained efficiently on Fractogel^® EMD TMAE Hicap (M) when the pH of the buffer is about 1 unit above their isoelectric points (pl).

The strength of the binding depends on the following:

• the buffer system
• pH value of the buffer which determines the surface charge of the protein
• the concentration of the counter ions
• the charge density on the support (protein binding capacity)

法律資訊

FRACTOGEL is a registered trademark of Merck KGaA, Darmstadt, Germany