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Merck
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Key Documents

1.25079

Millipore

MiniChrom Column Fractogel DEAE (M), 5 ml

MiniChrom Fractogel® DEAE (M), volume 5 mL, prepacked column

同義詞:

Fractogel® EMD DEAE (M)

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About This Item

分類程式碼代碼:
41115711

ligand

diethyl-(2-hydroxypropyl)aminoethyl

材料

polypropylene housing

產品線

Fractogel®

形狀

prepacked column

參數

≤8 bar pressure
~170 cm/hr max. flow rate

床長度

100 mm

床體積

5 mL

柱內徑

8 mm

容積

5 mL

基質活性組

Methacrylate

平均粒徑

40-90 μm

容量

~100 mg, BSA binding capacity

應用

gene therapy
mAb
vaccine development

分離技術

anion exchange

儲存溫度

15-25°C

一般說明

Weak cation exchange Fractogel® EMD DEAE (M) resin in a convenient pre-packed 5 ml column

特點和優勢

MiniChrom Column Fractogel® DEAE (M), 5 ml enables:
  • Excellent binding to large viruses and plasmid DNA
  • Homogenous binding with high selectivity and purity
  • Lower elution volumes for the highest purity levels
  • Compatibility with 2.5 % (v/v) aqueous benzyl alcohol containing 150 mM NaCl storage solution


Due to the titration behavior, the ion exchange capacity can be used from pH 2 to pH 9.5. The separation of proteins is based on reversible electrostatic interactions between the negatively charged regions of the proteins′ surface and the support. Proteins are retained efficiently on Fractogel® EMD DEAE when the pH of the buffer is about 1 unit above their isoelectric points (pl).

The strength of the binding depends on the following:
  • the buffer system
  • pH value of the buffer which determines the surface charge of the protein
  • the degree of the ionization of the functional groups of the exchanger
  • the concentration of the counter ions
  • the charge density on the support (protein binding capacity)

法律資訊

FRACTOGEL is a registered trademark of Merck KGaA, Darmstadt, Germany

分析證明 (COA)

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