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Merck
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Key Documents

12-500

Sigma-Aldrich

HeLa + EGF Stimulated Cell Lysate in Mg2+ Lysis Buffer

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About This Item

分類程式碼代碼:
41105330
eCl@ss:
32011807
NACRES:
NA.42

生物源

human

品質等級

形狀

liquid

製造商/商標名

Upstate®

技術

western blot: suitable

運輸包裝

dry ice

一般說明

EGF stimulated HeLa cell lysate. The cell lysate was quantitated via BCA assay. This sell lysate demonstrated higher levels of Ras-GTP form present as compared to unstimulated HeLa cell lysates. The assay was performed using the Ras GTPase Activation ELISA Kit (Catalog # 17-424)

細胞系描述

HeLa

外觀

100 &micor;g of EGF stimulated HeLa cell lysate, in 40 µL of 1X MLB (Catalog 20-168) containing, 1 &micor;g/mL leupeptin, 1µg/mL aprotinin, 1 µg/mL pepstatin, 1mM PMSF.

儲存和穩定性

Stable for 6 months at -80C from date of shipment. For maximum recovery of product, centrifuge the vial prior to removing the cap. Use immediately in assay following thawing as Ras GTP form can rapidly hydrolyze to the inactive Ras GDP form.

法律資訊

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

象形圖

Exclamation mark

訊號詞

Warning

危險聲明

危險分類

Eye Irrit. 2

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

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