1.05387

Leishman′s eosin methylene blue solution modified

for microscopy

• 分類程式碼代碼: 41116121
• NACRES: NA.05

屬性

• 蒸汽壓力: 128 hPa ( 20 °C)
• 品質等級: 400
• 形狀: liquid
• 自燃溫度: 455 °C (methanol)
• expl. lim.: 5.5-44 % (v/v) methanol()
• IVD: for in vitro diagnostic use
• bp: 65 °C/1013 hPa
• 轉變溫度: flash point 12 °C
• 密度: 0.79 g/cm³ at 20 °C
• 儲存溫度: 20-25°C

描述

一般說明

The staining method according to Leishman is one of the standard techniques in hematological diagnostic procedures. Like Wright′s eosin methylene blue solution and May-Grünwald′s eosin methylene blue solution,this ready-to-use solution is used for staining of blood and bone marrow smears and clinical cytological specimens. The typical resulting color of cell nuclei is red to purple, which depends on the buffer tablets (Buffer tablets pH 7.2 or Buffer tablets pH 6.8). The staining of the nuclei is due of the molecular interaction of the Eosin Y dye and a complex of Azur B with DNA. Both dyes assemble to an Eosin Y - Azur B-DNA complex and the intensity of the resulting stain depends on the content of Azur B and the ratio of Azur B : Eosin Y. Furthermore, the resulting stain can vary depending on the influence of fixation, staining times, pH-value of the solutions or buffer substances.
A 500 mL bottle of Leishman′s eosin methylene blue solution are sufficient for staining up to 1600 slides in Hellendahl cells. As an IVD product and CE registered, it can be used hassle-free in diagnostics and laboratory accreditations. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

應用

For microscopy. For staining of blood and bone marrow smears and clinical-cytological specimens

分析報告

Suitability for microscopy (Blood smear):passes test
Erythrocytes:reddish
Nuclei:violet
Eosinophilic granules:brick-red
Neutrophilic granules:light violet
Lymphocyte cytoplasm:blue

安全資訊

• 象形圖: GHS02,GHS06,GHS08
• 訊號詞: Danger
• 危險聲明: H225,H301 + H311 + H331,H317,H370
• 防範說明: P210 - P233 - P280 - P301 + P310 - P303 + P361 + P353 - P304 + P340 + P311
• 危險分類: Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Flam. Liq. 2 - Skin Sens. 1 - STOT SE 1
• 標靶器官: Eyes,Central nervous system
• 儲存類別代碼: 3 - Flammable liquids
• 水污染物質分類（WGK）: WGK 2
• 閃點（°F）: 53.6 °F
• 閃點（°C）: 12 °C