1.01287

Löffler′s methylene blue solution

for microscopy

• 同義詞: Löfflere′smethylene blue solution, Methylene blue solution acc. to Löffler
• 分類程式碼代碼: 41116130
• NACRES: NA.74

屬性

• 品質等級: 400
• 形狀: liquid
• IVD: for in vitro diagnostic use
• 技術: microbe id | staining: suitable
• pH值: 7.0-7.5 (20 °C in H₂O)
• 轉變溫度: flash point 40 °C
• 密度: 0.97 g/cm³ at 20 °C
• 應用: clinical testing; diagnostic assay manufacturing; hematology; histology
• 儲存溫度: 15-25°C

描述

一般說明

Löffler′s methylene blue solution - for microscopy, is a ready-to-use-solution and is used for human-medical cell diagnosis and serves the purpose of the bacteriological and histological investigation of sample material of human origin, for example smears of enriched bacterial cultures or histological sections of e. g. the lung. The cell wall of acid-fast bacteria has a high proportion of wax and lipids and hence absorbs dyes only very slowly. The most efficient staining method is the hot staining according to Ziehl-Neelsen. In this method, carbolfuchsin solution is applied to the specimen and heated. This heating process accelerates the rate at which the fuchsin dye is absorbed and thus also that of the formation of the mycolate-fuchsin complex in the cell wall. Once the acid-fast bacteria have absorbed the fuchsin dye, it is virtually impossible to decolorize them again, even when they are intensively treated with a decolorizing solution such as e. g. hydrochloric acid in ethanol. Accordingly, acid-fast bacteria are termed as acid- and alcohol-fast for staining, and are stained red in the microscopic visualization. Correspondingly, all non-acid-fast microorganisms are counterstained with an appropriate dye. In this instruction for use methylene blue is used for counterstaining.
Pretreatment of the specimens with Sputofluol^™ dissolves the bacteria from the surrounding viscid sputum and cell material.
The 500 ml bottle is sufficient for approx. 250 stainings. This product is registered as IVD and CE marked. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

法律資訊

SPUTOFLUOL is a trademark of Merck KGaA, Darmstadt, Germany

安全資訊

• 象形圖: GHS02
• 訊號詞: Warning
• 危險聲明: H226
• 防範說明: P210 - P233 - P240 - P241 - P242 - P243
• 危險分類: Flam. Liq. 3
• 儲存類別代碼: 3 - Flammable liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: 104.0 °F
• 閃點（°C）: 40 °C