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Merck
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Key Documents

1.00327

Sigma-Aldrich

氯化氢 – 乙醇 溶液

in ethanol, for microscopy

同義詞:

氯化氢乙醇溶液

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About This Item

線性公式:
HCl · CH3CH2OH
CAS號碼:
分子量::
36.46
Beilstein:
1098214
MDL號碼:
分類程式碼代碼:
41116130

品質等級

形狀

liquid

IVD

for in vitro diagnostic use

pH值

1.0 (20 °C in H2O)

轉變溫度

flash point 21 °C

密度

0.90 g/cm3 at 20 °C

應用

clinical testing
diagnostic assay manufacturing
hematology
histology

儲存溫度

2-30°C

SMILES 字串

Cl

InChI

1S/ClH/h1H

InChI 密鑰

VEXZGXHMUGYJMC-UHFFFAOYSA-N

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一般說明

Hydrochloric acid in ethanol - for microscopy, is used for human-medical cell diagnosis and serves the purpose of the bacteriological and histological investigation of sample material of human origin. Acid-fast bacteria are difficult to stain because of the high proportion of lipid and wax in their cell walls. The most efficient staining method is the hot staining according to Ziehl-Neelsen. In this method, carbolfuchsin solution is applied to the specimen and heated. This heating process accelerates the rate at which the fuchsin dye is absorbed and thus also that of the formation of the mycolatefuchsin complex in the cell wall. With the Kinyoun method, a modification of the AFB-Color carbol fuchsin solution renders heating unnecessary (cold staining). Once the acid-fast bacteria have absorbed the fuchsin dye, it is virtually impossible to decolorize them again, even when they are intensively treated with a decolorizing solution such as e. g. hydrochloric acid in ethanol (Product number 1.00327). Accordingly, acid-fast bacteria are termed as acid- and alcohol-fast for staining, and are stained red in the microscopic visualization. Correspondingly, all non-acid-fast microorganisms are counterstained with an appropriate dye. In this instruction for use malachite green is used for counterstaining.
Pretreatment of the specimens with Sputofluol dissolves the bacteria from the surrounding viscid sputum and cell material. It is an IVD and CE registered product and can be applied for diagnostical purposes. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

應用

For microscopy. Ziehl-Neelsen staining

法律資訊

SPUTOFLUOL is a trademark of Merck KGaA, Darmstadt, Germany

象形圖

FlameCorrosion

訊號詞

Danger

危險聲明

危險分類

Eye Irrit. 2 - Flam. Liq. 2 - Met. Corr. 1

儲存類別代碼

3 - Flammable liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

69.8 °F

閃點(°C)

21 °C


分析證明 (COA)

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