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Key Documents

09-818

Sigma-Aldrich

抗-CARM1抗体

from rabbit

同義詞:

Protein arginine N-methyltransferase 4, coactivator-associated arginine methyltransferase 1, coactivator-associated arginine methyltransferase-1

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About This Item

分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.41

生物源

rabbit

品質等級

抗體表格

affinity isolated antibody

抗體產品種類

primary antibodies

無性繁殖

polyclonal

物種活性

rat, human

物種活性(以同源性預測)

mouse

技術

western blot: suitable

NCBI登錄號

UniProt登錄號

運輸包裝

wet ice

目標翻譯後修改

unmodified

基因資訊

human ... CARM1(10498)
mouse ... Carm1(59035)

一般說明

CARM1(与共激活因子相关的精氨酸甲基转移酶1)是一种精氨酸N-甲基转移酶,可催化甲基从S-腺苷-L-蛋氨酸转移至蛋白质中的精氨酸残基。 CARM1目标包括Arg17处的组蛋白H3和Arg2142处的p300甲基化。基因激活后,CARM1与来自p300和p160家族的组蛋白乙酰转移酶一起被募集到启动子。在单核细胞炎症刺激过程中,CARM1与p300一起充当NF-kB的共激活因子。它负责在发育中的胚泡中将细胞移向内部细胞团。CARM1在雄激素受体中也起着重要作用,并可能在前列腺癌进展中起作用。

特異性

可识别CARM1,Mr ~60 kDa。

免疫原

含有序列(CSPMSIPTNTMHYGS)的KLH偶联的合成肽,对应于人CARM1的氨基酸595-608。
表位:氨基酸595-608

應用

抗CARM1抗体是一种兔多克隆抗体,用于检测CARM1(也称为蛋白质精氨酸N-甲基转移酶4,共激活因子相关的精氨酸甲基转移酶1),&已在WB中得到验证。
蛋白质印迹分析:
该批次的1 μg/mL稀释液在HeLa核提取物中检测到CARM1。(图A)。

標靶描述

~60 kDa

外觀

形式:纯化
通过肽亲和层析纯化。在0.1M Tris-甘氨酸(pH 7.4),15 mM NaCl和0.05% 叠氮化钠中以1 mg/ml提供。

分析報告

对照
包括阳性抗原对照:目录号12-309,HeLa核提取物。将等体积的Laemmli样品还原性缓冲液加至10 μL裂解液中,煮沸5分钟以对制剂进行还原。 在微凝胶每个泳道上样20 μg经还原的裂解液。

其他說明

浓度:请参考批次特异性浓缩物的分析证书。

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儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

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存取文件庫

Maria-Elena Torres-Padilla et al.
Nature, 445(7124), 214-218 (2007-01-12)
It has been generally accepted that the mammalian embryo starts its development with all cells identical, and only when inside and outside cells form do differences between cells first emerge. However, recent findings show that cells in the mouse embryo
Heng Hong et al.
Cancer, 101(1), 83-89 (2004-06-29)
Coactivator-associated arginine methyltransferase 1 (CARM1) is a transcriptional coactivator of the androgen receptor (AR). It is involved in the regulation of the biologic functions of the AR. It remains to be determined whether CARM1 is involved in prostatic carcinogenesis. The
D Chen et al.
Science (New York, N.Y.), 284(5423), 2174-2177 (1999-06-26)
The p160 family of coactivators, SRC-1, GRIP1/TIF2, and p/CIP, mediate transcriptional activation by nuclear hormone receptors. Coactivator-associated arginine methyltransferase 1 (CARM1), a previously unidentified protein that binds to the carboxyl-terminal region of p160 coactivators, enhanced transcriptional activation by nuclear receptors
Adam Burton et al.
Cell reports, 5(3), 687-701 (2013-11-05)
Cell plasticity or potency is necessary for the formation of multiple cell types. The mechanisms underlying this plasticity are largely unknown. Preimplantation mouse embryos undergo drastic changes in cellular potency, starting with the totipotent zygote through to the formation of
Cynthia M Quintero et al.
Journal of molecular biology, 430(21), 4168-4182 (2018-08-29)
Activation of the retinoic acid (RA) signaling pathway is important for controlling embryonic stem cell differentiation and development. Modulation of this pathway occurs through the recruitment of different epigenetic regulators at the retinoic acid receptors (RARs) located at RA-responsive elements

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