09-456

Anti-AIRE Antibody

Upstate^®, from rabbit

• 同義詞: APECED protein, Autoimmune polyendocrinopathy candidiasis ectodermal dystrophy ; ; protein, autoimmune polyendocrinopathy candidiasis ectodermal dystrophy 2 ; ; autoimmune regulator ; ; , autoimmune regulator (APECED protein), autoimmune
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: rabbit
• 品質等級: 100
• 抗體表格: affinity purified immunoglobulin
• 抗體產品種類: primary antibodies
• 無性繁殖: polyclonal
• 純化經由: affinity chromatography
• 物種活性: mouse
• 製造商／商標名: Upstate^®
• 技術: western blot: suitable
• 同型: IgG
• NCBI登錄號: NM_000383.2; NM_000658.1
• UniProt登錄號: O43918
• 運輸包裝: wet ice
• 目標翻譯後修改: unmodified
• 基因資訊: mouse ... Aire(11634)

描述

一般說明

AIRE (Autoimmune Regulator) is a human gene that is expressed primarily in the thymus. It causes transcription of a wide selection of organ-specific genes, reducing the threat of autoimmunity occurring by allowing the elimination of autoreactive T cells via negative selection. Defects in this gene cause the rare autosomal-recessive systemic autoimmune disease termed autoimmune polyendo-crinopathy-candidiasis-ectodermal dystrophy (APECED). Disruption of AIRE results in the development of a range of autoimmune diseases, the most common clinical conditions in the syndrome are hypoparathyroidism, primary adrenocortical failure and chronic mucocutaneous candidiasis. Aire functions through initiating the transcription of a diverse set of self-antigens, such as insulin, in the thymus. This expression then allows maturing thymocytes to become tolerant towards peripheral organs, thereby suppressing autoimmune disease. Aire is atranscriptional regulator protein that binds to DNA as dimer and tetramer, but not as a monomer. It forms nuclear bodies and interacts with the transcriptional coactivator CBP (CREBBP). At least three splice variant mRNAs products have been described including one which results in a premature stop codon and a transcript predicted to be a candidate for nuclear-mediated decay (NMD).

特異性

Likely to cross-react with rat, based on sequence homology. Cross-reactivity with other species has not been tested

Recognizes murine AIRE, Mr 58 kDa (calculated). Appears at ~65 kDa in Western blots.

免疫原

Epitope: a.a. 162-181

KLH-conjugated synthetic peptide corresponding to amino acids 162-181 of mouse AIRE

應用

Suggested dilutions:
Western blot: 1:1000

Detect AIRE using this Anti-AIRE Antibody validated for use in WB.

Research Category
Inflammation & Immunology

Research Sub Category
Developmental Signaling

Transcription Factors

RNA Binding Protein (RBP)

品質

Routinely evaluated by Western Blot analysis.

Western Blot:
A 1:1000 dilution of this lot detected AIRE in murine thymus lysate

標靶描述

65 kDa

外觀

ImmunoAffinity Purified

100 μg of immunoaffinity purified rabbit polyclonal IgG in 173 μl of 0.02M PBS, 0.25M NaCl containing 0.1% sodium azide

儲存和穩定性

2 years at -20°C from date of shipment. Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.

分析報告

Control
Murine thymus tissue lysate

法律資訊

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 2
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable