07-706

Anti-WDR5 Antibody

Upstate^®, from rabbit

• 同義詞: BIG3, BMP2-induced 3-kb gene protein, Cilia and flagella associated protein 89, Set1c WD40 repeat protein homolog, SWD3, WD repeat-containing protein 5, WD-repeat protein 5
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: rabbit
• 品質等級: 100
• 抗體表格: affinity purified immunoglobulin
• 抗體產品種類: primary antibodies
• 無性繁殖: polyclonal
• 純化經由: affinity chromatography
• 物種活性: human
• 製造商／商標名: Upstate^®
• 技術: western blot: suitable
• 同型: IgG
• NCBI登錄號: NP_060058
• UniProt登錄號: P61964
• 運輸包裝: dry ice
• 目標翻譯後修改: unmodified
• 基因資訊: human ... WDR5(11091)

描述

一般說明

WD repeat-containing protein 5 (also known as BMP2-induced 3-kb gene protein, WD repeat-containing protein 5, WD-repeat protein 5, Set1c WD40 repeat protein homolog, cilia and flagella associated protein 89) is a nuclear protein encoded by the WDR5 (also known as BIG3, CFAP89, SWD3) gene in human. This protein contains 7 WD (Trp-Asp) repeats and belongs to the WD repeat protein family whose members are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. WD repeat-containing protein 5 is a component of several complexes that regulate histone methylation and acetylation levels, including ATAC (ADA2A-containing complex) complex, SET1 complex, MLL1/MLL, MLL2/3 (also named ASCOM complex), MLL4/WBP7, and NSL (non-specific lethal) complex.

特異性

Evaluated by Western Blotting in HeLa nuclear extract.

Western Blotting Analysis: A 1:4,000 dilution of this antibody detected WDR5 in 10 µg of HeLa nuclear extract.

Recognizes WDR5, MW 36 kDa. A related protein band of 43 kDa that was blocked by the immunizing peptide was also detected. Identification of WDR5 protein at 36 kDa was confirmed with siRNA knockdown by an independent laboratory.

免疫原

Epitope: Near N-terminus

KLH-conjugated linear peptide corresponding to the N-terminus of human WDR5.

應用

Detect WDR5 using this Anti-WDR5 Antibody validated for use in WB.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histones

Western Blot: 0.05-0.25 μg/mL using HeLa nuclear extracts.

品質

Evaluated by Western Blotting in HeLa nuclear extract.

Western Blotting Analysis: A 1:4,000 dilution of this antibody detected WDR5 in 10 µg of HeLa nuclear extract.

標靶描述

~39 kDa observed

外觀

Affinity purified

Purified rabbit polyclonal IgG in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide with 30% glycerol.

儲存和穩定性

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

其他說明

Concentration: Please refer to lot specific datasheet.

法律資訊

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable