07-2162

Anti-PCNA Antibody

from rabbit

• 同義詞: proliferating cell nuclear antigen, cyclin, DNA polymerase delta auxiliary protein, Cyclin
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702

屬性

• 生物源: rabbit
• 品質等級: 100
• 抗體產品種類: primary antibodies
• 無性繁殖: polyclonal
• 物種活性: rat, mouse, human
• 物種活性（以同源性預測）: chimpanzee (based on 100% sequence homology)
• 技術: immunocytochemistry: suitable; western blot: suitable
• NCBI登錄號: NP_002583
• UniProt登錄號: P12004
• 運輸包裝: wet ice
• 目標翻譯後修改: unmodified
• 基因資訊: human ... PCNA(5111)

描述

一般說明

Proliferating cell nuclear antigen (PCNA) belongs to the DNA sliding clamp family and is comprised of three identical monomers forming a heterodimer. Due to its unique structure, PCNA is able to increase the processivity of DNA polymerase delta by using its outer domains to be involved in protein-protein interaction while still tethered to and freely sliding along the DNA. PCNA is a critical regulatory component in the onset of leading strand DNA replication and the termination of lagging strand synthesis. Studies have also indicated a role for PCNA in the recruitment and regulation of MMR pathway repair proteins. PCNA is also able to interact with a wide variety of cell cycle proteins and serves to coordinate several proteins participating in DNA processes, such as apoptosis.

特異性

Other homologies: Bovine, rabbit, canine, and panda (99% sequence homology).

免疫原

GST-tagged recombinant protein corresponding to human PCNA.

應用

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Cell Cycle, DNA Replication & Repair

Use Anti-PCNA Antibody (Rabbit Polyclonal Antibody) validated in WB, ICC to detect PCNA also known as proliferating cell nuclear antigen, cyclin, DNA polymerase delta auxiliary protein, Cyclin.

Western Blot Analysis: 1 µg/mL from a previous lot detected PCNA on 10 µg of NIH/3T3 and PC12 cell lysates.

Immunocytochemistry Analysis: 1:500 dilution from a previous lot detected PCNA in A431 cells.

品質

Evaluated by Western Blot in A431 cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected PCNA on 10 µg of A431 cell lysate.

標靶描述

~ 35 kDa observed. PCNA has a predicted molecular weight of 29 kDa, however, it can be monoubiquitinated, resulting in a ~ 35 kDa band.

外觀

Protein A

Format: Purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

分析報告

Control
A431 cell lysate

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable