07-2119

Anti-phospho-RalA Antibody (Ser194)

from rabbit, purified by affinity chromatography

• 同義詞: Ras-related protein Ral-A
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702

屬性

• 生物源: rabbit
• 品質等級: 100
• 抗體產品種類: primary antibodies
• 無性繁殖: polyclonal
• 純化經由: affinity chromatography
• 物種活性: human
• 物種活性（以同源性預測）: rat (based on 100% sequence homology), zebrafish (based on 100% sequence homology), bovine (based on 100% sequence homology), Xenopus (based on 100% sequence homology)
• 技術: inhibition assay: suitable (peptide); western blot: suitable
• NCBI登錄號: NP_005393
• UniProt登錄號: P11233
• 運輸包裝: wet ice
• 目標翻譯後修改: phosphorylation (pSer194)
• 基因資訊: human ... RALA(5898)

描述

一般說明

RalA and RalB are Ras-family small monomeric GTPases that can be activated through a Ras-dependent mechanism. The two are highly homologous (89%). Ral proteins are proximal effectors of oncogenic Ras and their activity is needed to support tumorigenesis in certain cancers. Mutants of Ras that are defective in activating all known Ras effectors except Ral GEF are still able to induce invasive phenotypes in transfected cells, suggesting a role for Ral in these processes. RalA is required for anchorage-independent proliferation and tumorigeneisis by driving exocyst assembly and polarized exocytosis in epithelial cells.

特異性

This antibody recognizes RalA phosphorylated at Ser194.

免疫原

KLH-conjugated linear peptide corresponding to human RalA phosphorylated at Ser194.

應用

Detect the phospho-RalA (Ser194) protein using this Anti-phospho-RalA (Ser194) validated for use in WB & Peptide Inhibition Assay.

Western Blot Analysis: A representative lot detected phospho-RalA (Ser194) in certain primary and metastatic cell lysates, and in certain unmatched tumor tissue lysate samples.

品質

Evaluated by Western Blot in PANC10.05 cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected phospho-RalA in PANC10.05 cell lysate. Peptide inhibition was observed with the addition of phospho peptides but not with the addition of non-phospho peptides.

標靶描述

~25 kDa observed. Uncharacterized bands may be observed at ~20 kDa, 100 kDa, and 130-170 kDa in some cell lysates.

分析報告

Control
PANC10.05 cell lysate

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable