07-164-AF647

Anti-phospho H2A.X (Ser139) Antibody, Alexa Fluor^™ 647 Conjugate

from rabbit, ALEXA FLUOR^™ 647

• 同義詞: Histone H2A.X, Histone H2A.X, H2a/x
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: rabbit
• 品質等級: 100
• 共軛: ALEXA FLUOR^™ 647
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: polyclonal
• 物種活性: human
• 物種活性（以同源性預測）: vertebrates (based on 100% sequence homology)
• 技術: immunocytochemistry: suitable
• 同型: IgG
• NCBI登錄號: NP_002096
• UniProt登錄號: P16104
• 運輸包裝: wet ice
• 目標翻譯後修改: phosphorylation (pSer139)
• 基因資訊: human ... ATP10D(57205) , H2AX(3014)

描述

一般說明

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. H2A.X, also known as H2AFX, is a variant of Histone H2A that is found in a subset of nucleosomes. H2A.X has a unique C-terminal tail that becomes phosphorylated following double-stranded DNA breaks following irradiation or apoptosis. Phosphorylation of H2A.X on Ser139 is one of the earliest markers for apoptosis and DNA damage.

特異性

Recognizes Histone H2A.X phosphorylated at Serine 139.

免疫原

Epitope: Phosphorylated Ser139

KLH-conjugated linear peptide corresponding to phospho H2A.X (Ser139).

應用

Research Category
Epigenetics & Nuclear Function

Epigenetics & Nuclear Function

Research Sub Category
Histones

Chromatin Biology

See data sheet for cat# 07-164, Anti-phospho H2A.X (Ser139) for additional Western blot, Immnoprecipitation, and ChIP-seq analysis performed using an unconjugated version of this antibody.

This AlexaFluor647 conjugated Anti-phospho Histone H2A.X antibody is validated for use in mmunocytochemistry for the detection of phospho-Histone H2A.X.

品質

Immunocytochemistry Analysis:

A 1:100 dilution of this antibody detected phospho Histone H2A.X (Ser139) in HeLa cells.
Alexa Fluor^™ is a registered trademark of Life Technologies.

標靶描述

~15 kDa observed

外觀

Protein A purified

Purified conjugated rabbit polyclonal antibody in buffer containing PBS and 15 mg/ml BSA with 0.1% Sodium Azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

其他說明

Concentration: Please refer to lot specific datasheet.

法律資訊

ALEXA FLUOR is a trademark of Life Technologies

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 2
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable