06-913-I

Anti-Sin3A Antibody

from rabbit, purified by affinity chromatography

• 同義詞: Paired amphipathic helix protein Sin3a, Histone deacetylase complex subunit Sin3a, Transcriptional corepressor Sin3a, Sin3A
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• 無性繁殖: polyclonal
• application: ICC; WB
• 物種活性: human, mouse
• 技術: immunocytochemistry: suitable; western blot: suitable

屬性

• 生物源: rabbit
• 品質等級: 100
• 抗體表格: affinity isolated antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: polyclonal
• 純化經由: affinity chromatography
• 物種活性: human, mouse
• 物種活性（以同源性預測）: rat (based on 100% sequence homology), rhesus macaque (based on 100% sequence homology)
• 技術: immunocytochemistry: suitable; western blot: suitable
• NCBI登錄號: NP_035508
• UniProt登錄號: Q60520
• 運輸包裝: wet ice
• 目標翻譯後修改: unmodified
• 基因資訊: human ... SAP30(8819) ; mouse ... Sin3A(20466)

描述

一般說明

Paired amphipathic helix protein Sin3a (UniProt Q60520; also known as Histone deacetylase complex subunit Sin3a, mSin3a, Transcriptional co-repressor Sin3a) is encoded by the Sin3A (also known as mSin3A, AW553200, Kiaa4126, mKIAA4126) gene (Gene ID 8819) in murine species. The transcription regulator Sin3a is a scaffold protein that interacts with various proteins to form specialized complexes that mediate different biological processes, including chromatin modification, embryonic development, DNA repair, mitochondrial metabolism, and gene transcription. SIN3a harbors four paired α-helices (PAH), a histone interaction domain, and a highly conserved region. The histone interaction domain mediates HDACs binding, whereas the highly conserved region interacts with proteins necessary for repression, such as the nuclear receptor hormone co-repressor Alien. The four PAHs represent the main interacting domains of SIN3a and mediate the interactions with a wide variety of DNA-binding transcription factors. Members of the Mad family of repressor proteins, for example, specifically interact with the PAH2 through their Sin3 interacting domain (SID). Although widely studied for its involvement in transcriptional repression, Sin3a is shown to be essential for aryl hydrocarbon receptor-mediated induction of gene transcription.

免疫原

Epitope: N-terminus

KLH-conjugated peptide (KRRLDDQESPVYAAQQRR-C) corresponding to amino acids 2-19 of mouse Sin3A.

應用

Anti-Sin3A Antibody is an antibody against Sin3A Antibody for use in Western Blotting and Immunocytochemistry.

Immunocytochemistry Analysis: 4.0 µg/mL from a representative lot detected Sin3A in HeLa, A431, HUVEC, and NIH/3T3 cells.

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

品質

Evaluated by Western Blotting in HeLa nuclear extract.

Western Blotting Analysis: 0.05 µg/mL of this antibody detected Sin3A in 10 µg of HeLa nuclear extract.

標靶描述

~160 kDa observed

聯結

Replaces: 06-913

外觀

Affinity purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

其他說明

Concentration: Please refer to lot specific datasheet.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable