推薦產品
生物源
rabbit
品質等級
抗體表格
serum
抗體產品種類
primary antibodies
無性繁殖
polyclonal
物種活性
human
技術
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
NCBI登錄號
UniProt登錄號
運輸包裝
wet ice
目標翻譯後修改
unmodified
基因資訊
human ... FCER1A(2205)
一般說明
The allergic reaction is mediated through the IgE high affinity receptor (FcεRI). When a multivalent antigen is presented, a redistribution of the monomeric IgE FcεRI receptor complexes causes the degranulation of mast cells and basophils, resulting in the subsequent release of factors responsible for the allergic reaction. The FcεRI receptor is a tetrameric complex, consisting of an a-chain, b-chain and a dimeric g-chain. The a-chain is primarily involved with the binding of IgE, but the signal is transferred through the g subunit, which contains the immunoreceptor tyrosine activation motifs (ITAM) critical for initiating receptor mediated signal transduction through the Syk and Lyn tyrosine kinases. The FcεRI receptor is able to engage and disengage the IgE, providing a versatile model for studying the function of kinase coupled receptors.
特異性
This antibody recognizes the extracellular domain of FcεRI α subunit.
免疫原
Epitope: Extracellular domain
GST-tagged recombinant protein corresponding to the extracellular domain of human FcεRI α subunit.
應用
Detect FcεRI α subunit using this Anti-FcεRI α subunit Antibody validated for use in Western Blotting, Immunoprecipitation, ICC.
Research Category
Inflammation & Immunology
Inflammation & Immunology
Research Sub Category
Inflammation & Autoimmune Mechanisms
Inflammation & Autoimmune Mechanisms
Western Blot Analysis: A 1:5,000 dilution from a representative lot detected FcεRI α subunit in 10 µg of human spleen tissue lysate.
Immunoprecipitation Analysis: A 1:50 dilution from a representative lot immunoprecipitated FcεRI α subunit from human spleen whole lysate.
Flow Cytometry Analysis: A 1:200 dilution from a representative lot detected FcεRI α subunit in RBL cells.
Immunoprecipitation Analysis: A 1:50 dilution from a representative lot immunoprecipitated FcεRI α subunit from human spleen whole lysate.
Flow Cytometry Analysis: A 1:200 dilution from a representative lot detected FcεRI α subunit in RBL cells.
品質
Evaluated by Western Blot in RBL cell lysate.
Western Blot Analysis: A 1:5,000 dilution of this antibody detected FcεRI α subunit in 10 µg of RBL cell lysate.
Western Blot Analysis: A 1:5,000 dilution of this antibody detected FcεRI α subunit in 10 µg of RBL cell lysate.
標靶描述
~57 kDa observed
聯結
Replaces: 06-725
外觀
Unpurified
Rabbit polyclonal serum containing 0.05% sodium azide.
儲存和穩定性
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
分析報告
Control
RBL cell lysate
RBL cell lysate
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
10 - Combustible liquids
水污染物質分類(WGK)
WGK 1
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號,寫有 ‘Lot’或‘Batch’.。
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