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重要文件

06-333

Sigma-Aldrich

Anti-MAPK1/ERK2 Antibody

Upstate, rabbit polyclonal

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About This Item

分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.41

產品名稱

Anti-MAP Kinase 2/Erk2 Antibody, Upstate®, from rabbit

生物源

rabbit

品質等級

抗體表格

purified antibody

抗體產品種類

primary antibodies

無性繁殖

polyclonal

物種活性

mouse, human, rat

製造商/商標名

Upstate®

技術

immunoprecipitation (IP): suitable
western blot: suitable

同型

IgG

NCBI登錄號

UniProt登錄號

運輸包裝

dry ice

目標翻譯後修改

unmodified

基因資訊

特異性

MAP Kinase encoded by the mapk gene

免疫原

Recombinant mouse ERK2

應用

Anti-MAP Kinase 2/Erk2 Antibody detects level of MAP Kinase 2/Erk2 & has been published & validated for use in IP & WB.
Research Category
Signaling
Research Sub Category
MAP Kinases

品質

routinely evaluated by immunoblot on mouse 3T3/A31 fibroblasts, human A431 carcinoma cells or rat L6 skeletal fibroblasts

標靶描述

42kDa

聯結

Replaces: 04-349

外觀

Protein G Chromatography
0.02M phosphate buffer, pH 7.6, 0.25M NaCl, 0.1% sodium azide
Format: Purified

儲存和穩定性

2 years at -20°C

分析報告

Control
Positive Antigen Control: Catalog #12-305, 3T3/A31 lysate. Add 2.5 μL of 2-mercapto-ethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.

法律資訊

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

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Dual phosphorylation and autophosphorylation in mitogen-activated protein (MAP) kinase activation.
Her, J H, et al.
The Biochemical Journal, 296 ( Pt 1), 25-31 (1993)
F Kiefer et al.
Molecular and cellular biology, 18(7), 4209-4220 (1998-06-25)
The cytoplasmic protein tyrosine kinase Syk has two amino-terminal SH2 domains that engage phosphorylated immunoreceptor tyrosine-based activation motifs in the signaling subunits of immunoreceptors. Syk, in conjunction with Src family kinases, has been implicated in immunoreceptor signaling in both lymphoid
Granulosa cell expression of G1/S phase cyclins and cyclin-dependent kinases in PMSG-induced follicle growth.
Jennifer D Cannon,Mary Cherian-Shaw,Tara Lovekamp-Swan,Charles L Chaffin
Molecular and cellular endocrinology null
J D Wright et al.
The Journal of biological chemistry, 270(20), 12085-12093 (1995-05-19)
Although signaling by the epidermal growth factor (EGF) receptor is thought to be dependent on receptor tyrosine kinase activity, it is clear that mitogen-activated protein (MAP) kinase can be activated by receptors lacking kinase activity. Since analysis of the signaling
S A Barber et al.
Journal of immunology (Baltimore, Md. : 1950), 155(3), 1404-1410 (1995-08-01)
LPS-stimulated macrophages (M phi) produce inflammatory mediators that are largely responsible for the pathophysiology associated with septic shock. M phi respond to LPS with rapid protein phosphorylation and dephosphorylation on serine, threonine, and tyrosine residues. If these events are critical

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