推薦產品
生物源
mouse
抗體表格
purified immunoglobulin
抗體產品種類
primary antibodies
無性繁殖
73P21, monoclonal
物種活性
human
製造商／商標名
Upstate®
技術
immunoprecipitation (IP): suitable
western blot: suitable
同型
IgG1
NCBI登錄號
UniProt登錄號
運輸包裝
wet ice
目標翻譯後修改
unmodified
基因資訊
human ... AKT1S1(84335)
一般說明
PRAS40 (Proline Rich Akt Substrate, 40 kDa), also known as AKT1S1, is a ubiquitously express protein that is phosphorylated on residue Thr246 via the PI3K/Akt pathway. It was originally discovered as an Akt substrate as it has the putative Akt phosphorylation motif of RxRxxpS/pT2. This phosphorylation allows it to bind 14-3-3 and Raptor of the mTOR complex mTORC1. PRAS40 is known to bind to and regulate mTORC1 (mTOR, Raptor, mLST8) kinases activity that is activated by insulin downstream of PI3K and Akt and subsequently phosphorylates p70S6K and 4EBP1. PRAS40 is known to have a putative TOS motif (FVMDE) that allows it bind to Raptor of mTORC1 in the absence of insulin1. It is thought that through it binding to the Raptor, PRAS40 inhibits the kinase activity of mTORC1 by preventing to bind to its substrates such as p70S6K and 4EBP1.
特異性
Recognizes PRAS40.
This antibody does not cross react with mouse.
免疫原
Recombinant human PRAS40 (full-length) expressed in E. coli.
應用
Anti-Pras40 Antibody, Clone 73P21 is an antibody against Pras40 for use in IP & WB.
Immunoblot Analysis: A 01-1.0 μg/mL dilution of this lot detected PRAS40 in RIPA lysates of HeLa cells.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
PI3K, Akt, & mTOR Signaling
PI3K, Akt, & mTOR Signaling
品質
Routinely evaluated by western blot.
Western Blot Analysis:
A 0.1-1.0 µg/mL dilution of this lot detected PRAS40 in RIPA lysates of HeLa cells.
Western Blot Analysis:
A 0.1-1.0 µg/mL dilution of this lot detected PRAS40 in RIPA lysates of HeLa cells.
標靶描述
40 kDa
外觀
Affinity purified
Format: Purified
Purified mouse monoclonal IgG1 in buffer containing PBS with 1% BSA and 0.1% sodium azide.
儲存和穩定性
Stable for 1 years at -20°C from date of receipt.
Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
分析報告
Control
RIPA lysates of HeLa cells.
RIPA lysates of HeLa cells.
其他說明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律資訊
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 2
閃點（°F）
Not applicable
閃點（°C）
Not applicable
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。
Evaluating the therapeutic potential of mTOR inhibitors using mouse genetics.
Huawei Li,Jennifer L Cotton,David A Guertin
Methods in Molecular Biology null
Daniel W D West et al.
The American journal of clinical nutrition, 94(3), 795-803 (2011-07-29)
Ingestion of whey or casein yields divergent patterns of aminoacidemia that influence whole-body and skeletal muscle myofibrillar protein synthesis (MPS) after exercise. Direct comparisons of the effects of contrasting absorption rates exhibited by these proteins are confounded by their differing
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