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重要文件

05-829-AF647

Sigma-Aldrich

Anti-α-Tubulin Antibody, clone DM1A, Alexa Fluor 647 conjugate

clone DM1A, from mouse, ALEXA FLUOR 647

同義詞:

Tubulin alpha-1 chain, α-Tubulin

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About This Item

分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.41

生物源

mouse

品質等級

共軛

ALEXA FLUOR 647

抗體表格

purified immunoglobulin

抗體產品種類

primary antibodies

無性繁殖

DM1A, monoclonal

物種活性

guinea pig, rat, porcine, human, gerbil, mouse, bovine, avian

技術

immunocytochemistry: suitable

同型

IgG1

NCBI登錄號

UniProt登錄號

運輸包裝

wet ice

目標翻譯後修改

unmodified

基因資訊

human ... TUBA1A(7846)

一般說明

Tubulin alpha-1 chain (UniProt P02552) is encoded by the TUBA1C (also known as TUBA1) gene (Gene ID 429035) in Gallus gallus (Chicken) species. Microtubules are cytoskeletal filaments composed of alpha-/beta-tubulin heterodimers that self-assemble head-to-tail to form protofilaments and laterally to form a hollow tube. Various post-translational modifications, including acetylation, polyglutamylation, polyglycylation, detyrosination, phosphorylation, and palmitoylation, can regulate the polymerization properties of tubulins and/or their interactions with microtubule associated proteins (MAPs) and motor proteins.

特異性

Clone DM1A recognizes α-Tubulin, MW 50-60 kDa. The epitope recognized by clone DM1A has been mapped to the C-terminal 25 amino acids.

免疫原

Epitope: C-terminal 25 amino acids.
Purified chicken brain α-Tubulin. The epitope recognized by this antibody has been mapped to the C-terminal 25 amino acids (AALEKDYEEVGVDSVEGEGEEEGEE).

應用

Anti-α-Tubulin Antibody, clone DM1A, Alexa Fluor 647 conjugate is an antibody against α-Tubulin for use in Immunocytochemistry.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected α-Tubulin in NIH/3T3 cells.
The unconjugated antibody (Cat. No. 05-829) is shown to be suitable also for immunocytochemistry, immunofluorescence, and Western blotting applications.
Research Category
Cell Structure
Research Sub Category
Cytoskeleton

品質

Evaluated by Immunocytochemistry in A431 cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected α-Tubulin in A431 cells.

標靶描述

~50 kDa observed.

外觀

Protein G Purified
Purified mouse monoclonal IgG1 antibody conjugate in PBS with 15 mg/mL BSA and 0.1 % sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

其他說明

Concentration: Please refer to lot specific datasheet.

法律資訊

ALEXA FLUOR is a trademark of Life Technologies

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

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Brandon Weissbourd et al.
Cell, 184(24), 5854-5868 (2021-11-26)
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Biomedical optics express, 14(1), 429-440 (2023-01-27)
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