05-516-AF647

Anti-Ras Antibody, clone RAS10, Alexa Fluor^™ 647 Conjugate

clone RAS10, from mouse, ALEXA FLUOR^™ 647

• 同義詞: GTPase HRas, c-H-ras, H-Ras-1, Ha-Ras, p21ras, Transforming protein p21, GTPase KRas, c-K-ras, c-Ki-ras, K-Ras 2, Ki-Ras, GTPase NRas, Transforming protein N-Ras
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702

屬性

• 生物源: mouse
• 品質等級: 100
• 共軛: ALEXA FLUOR^™ 647
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: RAS10, monoclonal
• 物種活性: mouse, rat, human
• 技術: flow cytometry: suitable; immunocytochemistry: suitable
• 同型: IgG2aκ
• NCBI登錄號: NM_001130442; NM_005343
• UniProt登錄號: P01112
• 運輸包裝: ambient
• 目標翻譯後修改: unmodified
• 基因資訊: human ... HRAS(3265) , KRAS(3845) , NRAS(4893)

描述

一般說明

Ras proteins are small GTPases controlling cellular pathways that signal for growth, proliferation, and differentiation. Mammalian genomes harbor three Ras genes (HRAS, NRAS, and KRAS) that encode four GTPases, two of which (K-Ras4A and K-Ras4B) are transcribed from the KRAS gene via two alternative fourth exons. Ras proteins share nearly identical sequence homology in sequence encoded by exons 1-3 (a.a. 1-165), while diverge in their C-terminal 24-a.a. hypervariable region (HVR) encoded by exon 4. The first 165 amino acids form the catalytic G domain that mediates GDP/GTP binding and association with effectors, exchange factors, and GTPase-activating proteins (GAPs), while the C-terminal HVR is needed for Ras membrane localization, a process necessary for Ras function. In addition to the removal of initiator methionine (Met1), Ras proteins are posttranslationally modified by farnesylation of Cys186, followed by proteolytical cleavage their C-terminal end three amino acids (a.a. 187-189) and carboxyl methylation of the newly formed C-terminal prenylcysteine. H-Ras, N-Ras, and K-Ras4A are further palmitoylated on cysteine residue(s) near the C-terminus (Cys181/Cys184 in H-Ras, Cys181 in N- and K-Ras), while K-Ras4B lacks a palmitoylation site. Palmitoylation is required for targeting H- and N-Ras to the plasma membrane (PM), while K-Ras4A and K-Ras4B contain a series of lysines that constitute a polybasic region (PBR) capable of interacting with the inner leaflet of the PM through an electrostatic interaction.

應用

Detect Ras using this mouse monoclonal Anti-Ras Antibody, clone RAS10, Alexa Fluor^™ 647 Conjugate, Cat. No. 05-516-AF647, validated for use in Flow Cytometry aned Immunocytochemistry.

Flow Cytometry Analysis: 0.2 µL from a representative lot detected Ras immunoreactivity in one million A431 cells.

Clone Ras10 is also available as unconjugated, as well as in FITC- and Alexa Fluor^™ 555-conjugated forms (Cat. Nos. 05-516, FCMAB148F, 05-516-AF555) for ELISA, flow cytometry, immunocytochemistry, immunohistochemistry, immunoprecipitation, and Western blotting applications.

品質

Evaluated by Immunocytochemistry in A431 cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Ras immunoreactivity in A431 cells.

標靶描述

~21 kDa observed. 21.30 kDa (human/mouse/rat H-Ras4A, p21 pro-form), 20.87 kDa (human/mouse/rat mature H-Ras4A, p21), 21.66 kDa (human/mouse/rat K-Ras4A pro-form), 21.17 kDa (human/mouse/rat mature K-Ras4A), 21.23/21.20/21.24 kDa (human/mouse/rat N-Ras pro-form), 20.90/20.90/20.91 kDa (human/mouse/rat mature N-Ras) calculated.

外觀

Purified mouse IgG2a Alexa Fluor^™ 647 conjugate in buffer containing PBS with 1.5% BSA and 0.05% sodium azide.

其他說明

Concentration: Please refer to lot specific datasheet.

法律資訊

ALEXA FLUOR is a trademark of Life Technologies

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 2
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable