05-1320-M

Anti-EED Antibody, clone AA19

ascites fluid, from mouse

• 同義詞: WD protein associating with integrin cytoplasmic tails 1, embryonic ectoderm development
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: mouse
• 品質等級: 100
• 抗體表格: ascites fluid
• 抗體產品種類: primary antibodies
• 無性繁殖: monoclonal
• 物種活性: human, mouse
• 技術: western blot: suitable
• 同型: IgG2aκ
• NCBI登錄號: NP_068676
• UniProt登錄號: O75530
• 目標翻譯後修改: unmodified
• 基因資訊: human ... EED(8726) ; mouse ... Eed(13626)

描述

一般說明

Polycomb group proteins are important for maintaining transcriptional silencing. One conserved PcG complex, PRC2, is composed of several proteins including the histone methyltransferase EZH2, the WD-repeat protein EED (Embryonic ectoderm development), and the Zn-finger protein Suz12. Transcriptional repression mediated by EED involves histone deacetylation, while the EZH2 methylates histone H3 on lysine 27. EED protein is present in four isoforms. These EED isoforms selectively associate with distinct EZH2-containing complexes, resulting in differential targeting of their associated methyltransferase activity. These complexes play a role in Hox gene silencing, X-inactivation, germline development, stem cell pluripotency and cancer metastasis. EED is also known to bind H3K27me3 and can allosterically stimulate the methyltransferase activity of the PRC2 complex. (Ref.: Hojfeldt, JW. et al (2018) Nat. Struc. Mol. Biol. 25 (3); 225-232).

特異性

Not tested in other species.

This antibody recognizes mouse EED, (~30-65 kDa).

免疫原

Recombinant fusion protein of full length murine EED tagged with MBP.

應用

Anti-EED Antibody, clone AA19 is a mouse monoclonal antibody for detection of EED also known as WD protein associating with integrin cytoplasmic tails 1 ,embryonic ectoderm development & has been validated in WB.

Research Category
Epigenetics & Nuclear Function

Apoptosis & Cancer

Research Sub Category
Chromatin Biology

品質

Routinely evaluated by Western Blot on A431cel lysatel.

Western Blot Analysis: 1:5,000 of this lot detected EED on 15 μg/mL of A431 cell lysate.

標靶描述

Ranges from 30-65 kDa (5-6 isoforms of EED are detected).

外觀

Protein G Purified

Purified immunoglobulin in 0.1 M Tris-Glycine (pH7.4) 150 mM NaCl with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.
For maximum recovery of product, centrifuge the vial prior to removing the cap. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

分析報告

Control
A431 Cell Lysate

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable