05-1139

Anti-Focal Adhesion Kinase Antibody, clone BLAb2H7

clone BLAb2H7, from mouse

• 同義詞: FADK 1, PTK2 protein tyrosine kinase 2, Protein-tyrosine kinase 2, focal adhesion kinase 1
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: mouse
• 品質等級: 100
• 抗體表格: purified immunoglobulin
• 抗體產品種類: primary antibodies
• 無性繁殖: BLAb2H7, monoclonal
• 物種活性: human, mouse, rat, chicken
• 技術: immunoprecipitation (IP): suitable; western blot: suitable
• 同型: IgG1
• NCBI登錄號: NP_990766.1
• UniProt登錄號: Q05397
• 運輸包裝: wet ice
• 目標翻譯後修改: unmodified
• 基因資訊: chicken ... Ptk2(396416) ; human ... PTK2(5747) ; mouse ... Ptk2(14083) ; rat ... Ptk2(25614)

描述

一般說明

FAK is a 125 Kd protein that is recruited as a participant in focal adhesion dynamics between cells and has a role in motility and cell survival. FAK is a highly conserved, non-receptor tyrosine kinase originally identified as a substrate for the oncogene protein tyrosine kinase v-src (Guan, J L and Shalloway, D). This cytosolic kinase has been implicated in diverse cellular roles including cell locomotion, mitogen response and cell survival. FAK is typically located at structures known as focal adhesions, these are multi-protein structures that link the extracellular matrix (ECM) to the cytoplasmic cytoskeleton. Additional components of focal adhesions include actin, filamin, vinculin, talin, paxillin and tensin (Burridge, K, et al).

特異性

This antibody recognizes Focal Adhesion Kinase

免疫原

Epitope: Terminal

Recombinant fragment derived from the amino terminal region of chicken FAK, amino acid residues 103 to 553.

應用

Immunohistochemistry Analysis: A previous lot detected Focal Adhesion Kinase at 1:100 dilution in mouse cortex tissue.

Research Category
Cell Structure

Research Sub Category
Cytoskeletal Signaling

This Anti-Focal Adhesion Kinase Antibody, clone BLAb2H7 is validated for use in WB, IP for the detection of Focal Adhesion Kinase.

品質

Evaluated by Western Blotting in HEK293 cell lysate.
Western Blotting Analysis: 0.2 µg/mL of this antibody detected Focal Adhesion Kinase in 10 µg of HEK293 cell lysate.

標靶描述

~125 kDa

聯結

Replaces: 04-591

外觀

Format: Purified

Purified mouse monoclonal IgG1 in buffer containing 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

分析報告

Control
HEK293 cell lysate

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: nwg
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable