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重要文件

04-903

Sigma-Aldrich

Anti-GSK3α/β Antibody, rabbit monoclonal

culture supernatant, from rabbit

同義詞:

GSK-3 alpha, glycogen synthase kinase 3 alpha

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About This Item

分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.41

生物源

rabbit

品質等級

抗體表格

culture supernatant

抗體產品種類

primary antibodies

無性繁殖

monoclonal

物種活性

mouse, human, rat

技術

immunoprecipitation (IP): suitable
western blot: suitable

同型

IgG

UniProt登錄號

運輸包裝

dry ice

目標翻譯後修改

unmodified

基因資訊

human ... GSK3B(2932)

一般說明

Glycogen Synthase Kinase 3 differs from most serine/threonine kinases in that it is active in the absence of the action of signaling pathways. Two isoforms exist, GSK3α and GSK3β. The function of GSK3 is to phosphorylate Glycogen Synthase and thereby inactivate it. Insulin action stimulates the PI3 Kinase pathway, resulting in Akt activation, which phosphorylates and inactivates GSK3. Glycogen Synthase is then rapidly dephosphorylated and activated. Other GSK3 substrates include Jun (on inhibitory sites), and eIF2B. Phosphorylation of Tau by GSK3 may relate to development of Alzheimer′s disease. Phosphorylation state-specific antibodies directed against the Akt site (Ser21) on GSK3 are suitable for surrogate assays of the activation state of the pathway.

特異性

GSK3 alpha and beta.
Predicted to cross-react with rat based on sequence homology.

免疫原

N-terminal 6His-tagged recombinant full length human GSK3β.

應用

Immunoprecipitation: 2-8 μL of a previous lot of antibody immunoprecipitated GSK3 alpha and beta from 100 μg of HEK293 RIPA lysate.
NOTE: This antibody also successfully immunoprecipitated GSK3 alpha and beta using the Catch and Release v2.0 Reversible Immunoprecipitation System (Catalog # 17-500).
Research Category
Signaling
Research Sub Category
PI3K, Akt, & mTOR Signaling
This Anti-GSK3α/β Antibody is validated for use in WB, IP for the detection of GSK3α/β.

品質

Evaluated by western blot on lysates from HEK293 cells.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected GSK3 alpha and GSK3 beta in RIPA lysates from A431, HEK293, Jurkat, HeLa NE or 3T3/A31 cells.

標靶描述

~46 kDa (beta) and 51 kDa (alpha)

聯結

Replaces: 05-903

外觀

Cultured supernantant containing 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

分析報告

Control
HEK293 cell lysate

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

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Johannes Menzel-Severing et al.
Scientific reports, 8(1), 10268-10268 (2018-07-08)
Understanding transcription factor (TF) regulation of limbal epithelial stem/progenitor cells (LEPCs) may aid in using non-ocular cells to regenerate the corneal surface. This study aimed to identify and characterize TF genes expressed specifically in LEPCs isolated from human donor eyes

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