04-841
Anti-ROKα/ROCK-II Antibody, clone A9W4, rabbit monoclonal
culture supernatant, clone A9W4, from rabbit
同義詞:
RhoA-binding kinase 2, Rho-associated, coiled-coil containing protein kinase 2, Rho-associated, coiled-coil-containing protein kinase II, p150 ROK-alpha, p164 ROCK-2, ROCK-II, ROKalpha, Rock2
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About This Item
分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.41
無性繁殖:
A9W4, monoclonal
application:
IP
WB
WB
物種活性:
rat, human, mouse, bovine
技術:
immunoprecipitation (IP): suitable
western blot: suitable
western blot: suitable
citations:
4
生物源
rabbit
品質等級
抗體表格
culture supernatant
抗體產品種類
primary antibodies
無性繁殖
A9W4, monoclonal
物種活性
rat, human, mouse, bovine
技術
immunoprecipitation (IP): suitable
western blot: suitable
NCBI登錄號
UniProt登錄號
運輸包裝
dry ice
目標翻譯後修改
unmodified
基因資訊
rat ... Rock2(25537)
一般說明
Rho-Associated Kinase (Rho-Kinase or ROK) has been classified into two isoforms: ROKα/ROCK-II and ROKβ/ROCK-I. These large cellular proteins (150-160 kDa), have been reported to be involved in the formation of stress fibers, focal adhesions and cardiac hypertonic responses. Current research suggests that ROK of smooth muscle may be involved in the contractile process via phosphorylation of MYPT1 and Myosin.
特異性
Predicted to cross-react with mouse and bovine based on sequence homology.
Recognizes ROKα/ROCK-II, Mr 150 kDa. Does not recognize ROKβ/ROCK-I. A non-specific band may also be detected, Mr 80 kDa.
免疫原
6His-tagged fusion protein corresponding to residues 2-543 of rat ROKα/ROCK-II.
應用
Detect ROKα/ROCK-II using this Anti-ROKα/ROCK-II Antibody, clone A9W4 validated for use in IP & WB.
Immunoprecipitation:
4 μL of a previous lot immunoprecipitated ROKα/ROCK-II from 500 μg of PC-12 lysate.
4 μL of a previous lot immunoprecipitated ROKα/ROCK-II from 500 μg of PC-12 lysate.
Research Category
Signaling
Signaling
Research Sub Category
Cytoskeletal Signaling
Cytoskeletal Signaling
品質
Routinely evaluated by Western BLot on RIPA lysates from PC-12 cells.
Western Blot Analysis: A 1:500-1:5000 dilution of this antibody detected ROKα/ROCK-II in RIPA lysates from PC-12 cells.
Western Blot Analysis: A 1:500-1:5000 dilution of this antibody detected ROKα/ROCK-II in RIPA lysates from PC-12 cells.
標靶描述
150 kDa
聯結
Replaces: 05-841
外觀
Cultured supernatant containing 0.05% sodium azide.
儲存和穩定性
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
分析報告
Control
RIPA lysates from PC-12 cells
RIPA lysates from PC-12 cells
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。
T Leung et al.
Molecular and cellular biology, 16(10), 5313-5327 (1996-10-01)
The GTPase RhoA has been implicated in various cellular activities, including the formation of stress fibers, motility, and cytokinesis. We recently reported on a p150 serine/threonine kinase (termed ROK alpha) binding RhoA only in its active GTP-bound state and on
S Vincent et al.
Molecular and cellular biology, 17(4), 2247-2256 (1997-04-01)
The Ras-related Rho family GTPases mediate signal transduction pathways that regulate a variety of cellular processes. Like Ras, the Rho proteins (which include Rho, Rac, and CDC42) interact directly with protein kinases, which are likely to serve as downstream effector
Ines Jeric et al.
Nature communications, 7, 13781-13781 (2016-12-22)
Hepatocellular carcinoma (HCC) is a leading cause of cancer deaths, but its molecular heterogeneity hampers the design of targeted therapies. Currently, the only therapeutic option for advanced HCC is Sorafenib, an inhibitor whose targets include RAF. Unexpectedly, RAF1 expression is
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