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重要文件

850563P

Avanti

Bicelle Kit

Avanti Research - A Croda Brand

同義詞:

Bicelle Creation Kit for High-quality Membrane Protein Crystal Production

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About This Item

分類程式碼代碼:
12352211
NACRES:
NA.28

一般說明

Bicelle kit contains 200mg of dimyristoylphosphatidylcholine (DMPC), 200mg of dimyristoylphosphatidylglycerol (DMPG), 1g of 3-[(3-cholamidopropyl)-dimethylammonio]-1-propane sulfonate (CHAPS) and 1g of 3-(cholamidopropyl)dimethylammonio-2-hydroxy-1-propane-sulfonate (CHAPSO). Bicelle is a discoidal lipid aggregate. A bicelle consists of a planar bilayer at the center made of long-chain phospholipids, which are encircled by a detergent layer. The planar bilayer is generally composed of DMPC, which may have phospholipids such as (DMPG) for alteration of charge of the membrane. The detergent layer is composed of bile salt derivative, such as CHAPSO.
The bicelle kit is designed to aid with the production of high-quality membrane protein crystals. Bicelles can be integrated into standard crystallization protocols, and in contrast to micelles, bicelles maintain the protein in a more native bilayer environment allowing proteins to be captured in a more biologically relevant orientation.

生化/生理作用

Bicelles acts as membrane mimetics and facilitate membrane-associated biomolecules study.

包裝

20ML Clear Glass Screw Cap Vial for each component (850563P-1EA)
Package contains 200MG of DMPC, 200MG of DMPG, 1G of CHAPS, and 1G of CHAPSO

法律資訊

Avanti Research is a trademark of Avanti Polar Lipids, LLC

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訊號詞

Warning

危險聲明

危險分類

Acute Tox. 4 Oral

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 3


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Crystallization of Membrane Proteins in Bicelles
Sayeh Agah; Salem Faham
Methods in Molecular Biology, 914 (2012)
K J Glover et al.
Biophysical journal, 81(4), 2163-2171 (2001-09-22)
Several complementary physical techniques have been used to characterize the aggregate structures formed in solutions containing dimyristoylphosphatidylcholine (DMPC)/dihexanoylphosphatidylcholine (DHPC) at ratios of < or =0.5 and to establish their morphology and lipid organization as that of bicelles. (31)P NMR studies

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