L040030

Cap A

configured for ABI

• 分類程式碼代碼: 12352200
• NACRES: NA.21

屬性

• 種類: for DNA synthesis
• 產品線: Proligo Reagents
• 形狀: liquid
• 儲存期限: 365 days
• 濃度: 97.0-119.0 g/L (Acetic Anhydride content, titration)
• 顏色: clear colorless, to brownish
• 相容性: configured for ABI
• 儲存溫度: 2-8°C

描述

一般說明

Cap A solution contains acetic anhydrideanhydride, lutidine and tetrahydrofuran (THF). It is used after the phosphoramidite reaction/coupling step. The solution is configured for ABI.

生化／生理作用

Cap A solution aborts chains bearing unreacted 5′ hydroxyl groups due to failure of reaction with activated phosphoramidite (typically, 1 to 2% of growing oligonucleotide chains in each synthesis cycle). These unreacted 5′ hydroxyl groups are capped with an acetyl group and thus rendered unreactive for subsequent synthesis steps.

準備報告

四氢呋喃/二甲基吡啶/乙酸酐 8/1/1 (v/v/v)

安全資訊

• 象形圖: GHS02,GHS06,GHS08,GHS05
• 訊號詞: Danger
• 危險聲明: H225,H302,H315,H318,H331,H335,H336,H351
• 防範說明: P210 - P280 - P301 + P312 - P304 + P340 + P311 - P305 + P351 + P338 - P308 + P313
• 危險分類: Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Carc. 2 - Eye Dam. 1 - Flam. Liq. 2 - Skin Irrit. 2 - STOT SE 3
• 標靶器官: Respiratory system
• 安全危害: EUH019
• 儲存類別代碼: 3 - Flammable liquids
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: 6.1 °F
• 閃點（°C）: -14.4 °C