907316

GlcA-ABP

95%

• 同義詞: (2S,3S,4S,5R,6S)-6-(2-(difluoromethyl)-4-(hex-5-ynamido)phenoxy)-3,4,5-trihydroxytetrahydro-2H-pyran-2-carboxylic acid, Glucuronic acid activity-based probe, Glucuronidated metabolite mimic
• 經驗公式（希爾表示法）: C₁₉H₂₁F₂NO₈
• 分子量：: 429.37
• 分類程式碼代碼: 12352101

屬性

• 化驗: 95%
• 形狀: solid
• 儲存溫度: −20°C

描述

應用

This glucuronic acid activity-based probe (GlcA-ABP) is a glucuronidated metabolite mimic that provides in situ detection of active β-glucuronidases in the gut microbiome. The probe reacts with nucleophilic residues of the target, and the alkyne tag enables subsequent bioorthogonal tagging via click chemistry for enrichment (i.e. biotin tags) or imaging and cell sorting (i.e. fluorescent tags). With GlcA-ABP, Whidbey, et al were able to pair the activity-based probe (ABP) with fluorescence-activated cell sorting (FACS; ABP-FACS) to identify β-glucuronidase-active taxa in the gut microbiota.

Currently in gut microbiome research, access to and differentiation between subpopulations and their relationships to host biochemistry is a challenge. Functional and chemical approaches such as those with GlcA-ABP provide a complementary tool to gene sequencing.

其他說明

A Probe-Enabled Approach for the Selective Isolation and Characterization of Functionally Active Sub populations in the Gut Microbiome

安全資訊

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable