一般說明
PLAM- AβIδ1LVproSTγ-13CH3 kit has 13CH3 isotopomer precursors and contains protocol instructions for creation of isotopically-labeled proteins.
應用
For detection of long-range nOes or for the NMR study of large proteins
PLAM- AβIδ1LVproSTγ-13CH3 is an optimized combinatorial kit which incorporates a 13CH3 isotopomer on the methyl groups of alanine, isoleucine, leucine and valine residues. This kit has been tested with protein isotopic labeling in E. coli. It is a combinatorial labeling kit which can be used in the detection of long-range NOEs between methyl probes up to 10 Å apart in large protein complexes.
包裝
This product may be available from bulk stock and can be packaged on demand. For information on pricing, availability and packaging, please contact Stable Isotopes Customer Service.
Rime Kerfah et al.
Current opinion in structural biology, 32, 113-122 (2015-04-17)
Nuclear magnetic resonance (NMR) spectroscopy is a uniquely powerful tool for studying the structure, dynamics and interactions of biomolecules at atomic resolution. In the past 15 years, the development of new isotopic labeling strategies has opened the possibility of exploiting
Silke Wiesner et al.
Current opinion in structural biology, 35, 60-67 (2015-09-26)
Intermolecular interactions are indispensible for biological function. Here we discuss how novel NMR techniques can provide unique insights into the assembly, dynamics and regulation of biomolecular complexes. We focus on applications that exploit the methyl TROSY effect and show that
文章
Methyl Isotope-Labeling of Proteins from NMR-Bio
Methyl groups are ideal probes for studying protein structure and function by NMR. Choose among user-friendly kits for isotope labeling of a single type or multiple types of methyl groups.
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