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G005102

GC Analysis of Proline Methyl Ester Enantiomers (N-Acetyl Derivatives) on Astec® CHIRALDEX™ G-TA

suitable for GC

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technique(s)

gas chromatography (GC): suitable

test parameters

column: Astec CHIRALDEX G-TA, 30 m x 0.25 mm I.D., 0.12 μm (73033AST)
oven: 155 °C
inj. temp.: 250 °C
detector: FID, 250 °C
carrier gas: helium, 30 psi
injection: 1 μL, 80:1 split
sample: 3 mg/mL racemate in methanol
peak 1: D-proline methyl ester (N-acetyl derivative)
peak 2: L-proline methyl ester (N-acetyl derivative

application(s)

food and beverages
life science and biopharma

Analysis Note

Elution order reversal using the N-trifluoroacetyl derivative.

Legal Information

Astec is a registered trademark of Merck KGaA, Darmstadt, Germany
CHIRALDEX is a trademark of Sigma-Aldrich Co. LLC

analytical column

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standard

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