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Key Documents

SRP8014

Sigma-Aldrich

Progranulin human

recombinant, expressed in HEK 293 cells, untagged, >95% (SDS-PAGE)

Synonym(s):

Activation-inducible TNF-related ligand, PEPI, Proepithelin

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About This Item

UNSPSC Code:
12352202

biological source

human

recombinant

expressed in HEK 293 cells

conjugate

untagged

Assay

>95% (SDS-PAGE)

form

lyophilized

mol wt

~65 kDa by SDS-PAGE

packaging

pkg of 10 μg

storage condition

avoid repeated freeze/thaw cycles

impurities

<0.1 EU/μg endotoxin, tested

color

white

UniProt accession no.

P28799

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... GRN(2896)

General description

Progranulin (PGRN) is a cysteine-rich protein with 593-amino acids and has a molecular weight of ~68.5kDa. The PGRN gene, with one non-coding exon and 12 coding exons spanning 8kb of genomic DNA, is mapped to human chromosome 17q21. The encoded protein is characterized by seven granulin-like domains, which include 7.5 highly conserved tandem repeats of a rare 12 cysteinyl residues. PGRN is predominantly expressed in rapidly cycling epithelial cells, leukocytes, neurons, and chondrocytes.

Biochem/physiol Actions

Progranulin (PGRN) is a widely expressed pluripotent growth factor, which plays a crucial role in various processes such as development, cell cycle progression, cell motility, wound repair and inflammation. Additionally, It is also implicated in early embryogenesis, host defense, cartilage development and degradation. Mutations in the PGRN gene were found in cases of frontotemporal degeneration (FTLD). PGRN expression is associated with the development of various type of cancer such as breast cancer, ovarian carcinoma, and multiple myeloma. Overexpression of PGRN has been observed in neurodegenerative diseases such as microglia of Creutzfeldt-Jacob patients and in spinal cord of amyotrophic lateral sclerosis (ALS) patients.

Physical form

Lyophilized from 0.2 μm-filtered solution in PBS.

Reconstitution

Reconstitute with 100 μL deionized water.

Other Notes

Signal peptide and human progranulin (aa 1-593) is untagged. Reflects the native sequence with no additional aa.

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Mutations other than null mutations producing a pathogenic loss of progranulin in frontotemporal dementia.
van der Zee J
Human Mutation, 28(4), 416-416 (2007)
Progranulin in neurodegenerative disease.
Petkau TL and Leavitt BR2
Trends in Neurosciences, 37(7), 388-398 (2014)
Progranulin in frontotemporal lobar degeneration and neuroinflammation.
Ahmed Z
Journal of Neuroinflammation (2007)
The growth factor progranulin binds to TNF receptors and is therapeutic against inflammatory arthritis in mice.
Tang W
Science, 332(6028), 478-484 (2011)
Lorenz Thurner et al.
Digestive diseases and sciences, 59(8), 1733-1742 (2014-03-05)
Recently, we identified neutralizing autoantibodies against progranulin (PGRN) in a wide spectrum of rheumatic diseases including cases with enteropathic spondylarthritis. PGRN is a secreted protein with strong anti-inflammatory effects, believed to be mediated by the direct inhibition of TNF receptors
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