SML1094

CHIR 98014

≥98% (HPLC), powder, GSK-3 inhibitor

• Synonym(s): N-6-[2-[[4-(2,4-Dichlorophenyl)-5-(1H-imidazol-1-yl)-2-pyrimidinyl]amino]ethyl]-3-nitro-2,6-pyridinediamine, CHIR-98014
• Empirical Formula (Hill Notation): C₂₀H₁₇Cl₂N₉O₂
• CAS Number: 252935-94-7
• Molecular Weight: 486.31
• MDL number: MFCD10565922
• UNSPSC Code: 12352200
• PubChem Substance ID: 329825471
• NACRES: NA.77

Properties

• product name: CHIR 98014, ≥98% (HPLC)
• Assay: ≥98% (HPLC)
• form: powder
• color: white to brown
• solubility: DMSO: 0.5 mg/mL, clear (warmed)
• storage temp.: −20°C
• SMILES string: NC1=C(C=CC(NCCNC2=NC(C3=C(Cl)C=C(Cl)C=C3)=C(N4C=CN=C4)C=N2)=N1)[N+]([O-])=O
• InChI: 1S/C20H17Cl2N9O2/c21-12-1-2-13(14(22)9-12)18-16(30-8-7-24-11-30)10-27-20(29-18)26-6-5-25-17-4-3-15(31(32)33)19(23)28-17/h1-4,7-11H,5-6H2,(H3,23,25,28)(H,26,27,29)
• InChI key: MDZCSIDIPDZWKL-UHFFFAOYSA-N

Description

Application

CHIR 98014 has been used for the generation of small molecules neural progenitor cells and differentiation towards motor neurons. It has also been used as a Wnt/β-catenin pharmacological agonist in the cell-conditioned medium to perform chromatin immunoprecipitation (ChIP) studies in HT22 neurons.

Biochem/physiol Actions

CHIR 98014 is a glycogen synthase kinase-3 (GSK-3) inhibitor with IC₅₀ values of 0.65 nM and 0.58 nM for GSK3α and GSK3β, respectively. CHIR98014 is 500-fold to >10,000-fold selectivity for GSK-3 versus 20 other protein kinases tested, including closest homologs Cdc2 and ERK2.

Reconstitution

Reconstitute in DMSO at 0.5 mg/mL. Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Storage and Stability

The product in the original sealed container is quite stable. It should be stored at −20°C in the powdered format.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable