MAK118
Invertase Activity Assay Kit
sufficient for 100 colorimetric or fluorometric tests
Synonym(s):
β-fructofuranosidase Assay Kit
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About This Item
UNSPSC Code:
12161503
NACRES:
NA.84
Recommended Products
usage
sufficient for 100 colorimetric or fluorometric tests
application(s)
cosmetics
environmental
food and beverages
detection method
colorimetric
fluorometric
storage temp.
−20°C
General description
Invertase (β-fructofuranosidase) is an enzyme that catalyzes the hydrolysis of sucrose to fructose and glucose. Invertase is produced by certain plants, microorganisms, and honey bees.
Application
Invertase Assay Kit has been used for screening inhibition of recombinant invertase inhibitor protein from sugarcane.
Features and Benefits
Compatible with high-throughput handling systems.
Suitability
Suitable for the detection of invertase activity in biological and environmental samples and for the screening of invertase inhibitors.
Principle
In this kit, invertase activity is determined by a coupled enzyme assay in which invertase cleaves sucrose to glucose and fructose, resulting in a colorimetric (570 nm)/fluorometric (λex = 535/λem = 587 nm) product, proportional to the invertase activity present. One unit of Invertase is the amount of enzyme that will catalyze the formation of 1.0 μmole of glucose per minute at pH 4.5 under the assay conditions.
Storage Class Code
10 - Combustible liquids
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In sugarcane, invertase enzymes play a key role in sucrose accumulation and are also involved in futile reactions where sucrose is continuously degraded during the pre- and post-harvest period, thereby reducing sugar yield and recovery. Invertase inhibitor (INVINH) proteins play
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