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M9130

Sigma-Aldrich

4-Methylumbelliferyl-β-D-glucuronide hydrate

≥98% (HPLC), BioReagent, for identification of transformed plants

Synonym(s):

4-Methylumbelliferyl-β-D-glucopyranosiduronic acid, MUG

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About This Item

Empirical Formula (Hill Notation):
C16H16O9 · xH2O
CAS Number:
881005-91-0
Molecular Weight:
352.29 (anhydrous basis)
MDL number:
MFCD09039280
UNSPSC Code:
12352200
PubChem Substance ID:
329818083
NACRES:
NA.52

grade

for molecular biology

product line

BioReagent

Assay

≥98% (HPLC)

form

powder

solubility

pyridine: 50 mg/mL, clear, colorless to very faintly yellow

storage temp.

−20°C

SMILES string

O.CC1=CC(=O)Oc2cc(O[C@@H]3O[C@@H]([C@@H](O)[C@H](O)[C@H]3O)C(O)=O)ccc12

InChI

1S/C16H16O9.H2O/c1-6-4-10(17)24-9-5-7(2-3-8(6)9)23-16-13(20)11(18)12(19)14(25-16)15(21)22;/h2-5,11-14,16,18-20H,1H3,(H,21,22);1H2/t11-,12-,13+,14-,16+;/m0./s1

InChI key

URVSQZMOFUEQAW-YYHOVTOASA-N

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General description

MUG is a chemical commonly used for the detection of transformed plants. MUG shows low levels of fluorescence until treatment with glucuronidase (GUS) encoded in transformed plants. Transformed plants can be identified by their MUG fluorescence.

Application

Suitable for fluorescent detection of glucuronidase gene expression in plants.

Reconstitution

Product is soluble in molecular biology grade water at a concentration of 0.35 mg/ml.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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L J Moberg
Applied and environmental microbiology, 50(6), 1383-1387 (1985-12-01)
An assay procedure to screen for Escherichia coli in foods by using 4-methylumbelliferyl-beta-D-glucuronide (MUG) incorporated into lauryl tryptose (LST) broth was evaluated. The beta-glucuronidase produced by E. coli cleaves the MUG substrate to yield a fluorescent end product. E. coli-negative
Andrew G Kunihiro et al.
Molecular nutrition & food research, 64(14), e2000072-e2000072 (2020-06-09)
Curcumin prevents bone loss in resorptive bone diseases and inhibits osteoclast formation, a key process driving bone loss. Curcumin circulates as an inactive glucuronide that can be deconjugated in situ by bone's high β-glucuronidase (GUSB) content, forming the active aglycone.
Yuan Ruan et al.
Current biology : CB, 28(17), 2718-2729 (2018-08-28)
The capacity for sustained cell division within the plant meristem is a critical determinant of organ structure and performance. This capacity is diminished in mutants lacking the microtubule-associated protein CLASP and when brassinosteroid signaling is increased. Here, we discovered that
M C Thaller et al.
Journal of clinical microbiology, 26(4), 791-793 (1988-04-01)
A new selective, differential plating medium to screen the common gram-negative urinary tract pathogens is described. The medium combines adonitol fermentation, phenylalanine deaminase, and beta-glucuronidase tests and allows the indole and cytochrome oxidase tests to be performed directly from the
Matthew Ramon et al.
The Plant cell, 31(7), 1614-1632 (2019-05-28)
Energy homeostasis is vital to all living organisms. In eukaryotes, this process is controlled by fuel gauging protein kinases: AMP-activated kinase in mammals, Sucrose Non-Fermenting1 (SNF1) in yeast (Saccharomyces cerevisiae), and SNF1-related kinase1 (SnRK1) in plants. These kinases are highly
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