L9043

Lysostaphin from Staphylococcus simulans

recombinant, expressed in E. coli, lyophilized powder

• Synonym(s): Glycyl-glycine Endopeptidase
• CAS Number: 9011-93-2
• Enzyme Commission number: 3.4.24.75 (BRENDA, IUBMB)
• MDL number: MFCD00131556
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• biological source: bacterial (Streptomyces globisporus)
• Quality Level: 200
• recombinant: expressed in E. coli
• Assay: ≥90% (HPLC)
• form: lyophilized powder
• specific activity: ≥3000 unit/mg solid
• technique(s): molecular cloning: suitable
• suitability: suitable for sequence analysis test
• application(s): life science and biopharma
• shipped in: dry ice
• storage temp.: −20°C

Description

General description

Lysostaphin, a zinc metalloenzyme, is mainly secreted as a proenzyme. It acts as an anti-staphylococcal agent and is highly stable at pH 4 and 5°C.

Application

Lysostaphin from Staphylococcus simulans has been used in a study to assess molecular cloning, sequencing, and expression of lytM, a unique autolytic gene of Staphylococcus aureus. It has also been used in a study to investigate the sequence analysis of a Staphylococcus aureus gene encoding a peptidoglycan hydrolase activity.

Biochem/physiol Actions

A protein complex with highly specific lytic activity against Staphylococcus species, including Staphylococcus aureus.

Lysostaphin possesses a lytic action against Staphylococcus aureus, which is used to treat antibiotic-resistant staphylococcal infections. It has the properties of enzymes, such as glycylglycine endopeptidase, endo-β-N-acetyl glucosamidase and N-acetyl muramyl-L-alanine amidase. Lysostaphin is also used as a preservative in food industry and in clinical labs for rapid screening.

The antimicrobial activity of lysostaphin, a metalloendopeptidase expressed by Staphylococcus simulans, is enhanced by binding to the C′ terminus of the membrane-associated Trap protein of Staphylococcus aureus.

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H334
• Precautionary Statements: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves