HPA002560
Anti-SERPINA3 antibody produced in rabbit
Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
Synonym(s):
Anti-ACT, Anti-Alpha-1-antichymotrypsin precursor, Anti-Cell growth-inhibiting gene 24/25 protein
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About This Item
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biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
product line
Prestige Antibodies® Powered by Atlas Antibodies
form
buffered aqueous glycerol solution
species reactivity
human
enhanced validation
independent
Learn more about Antibody Enhanced Validation
technique(s)
immunoblotting: 0.04-0.4 μg/mL
immunohistochemistry: 1:1000-1:2500
immunogen sequence
QDKMEEVEAMLLPETLKRWRDSLEFREIGELYLPKFSISRDYNLNDILLQLGIEEAFTSKADLSGITGARNLAVSQVVHKAVLDVFEEGTEASAATAVKITLLSALVETRTIVRFNRPFLMIIVPTDTQNIFFMSKVTNPKQEGAPHR
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... SERPINA3(12)
General description
SERPINA3 (serpin family A member 3) is a widely expressed protein that belongs to the serpin superfamily. It is synthesized by hepatocytes, bronchial epithelial cells and neuronal cells. It consists of a highly conserved fold and three β-sheets along with ~8 α-helices. In addition to the α, β region, it has a solvent-exposed stretch of amino acids called as the reactive center loop (RCL).
Immunogen
Alpha-1-antichymotrypsin precursor recombinant protein epitope signature tag (PrEST)
Application
Anti-SERPINA3 antibody produced in rabbit, a Prestige Antibody, is developed and validated by the Human Protein Atlas (HPA) project . Each antibody is tested by immunohistochemistry against hundreds of normal and disease tissues. These images can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. The antibodies are also tested using immunofluorescence and western blotting. To view these protocols and other useful information about Prestige Antibodies and the HPA, visit sigma.com/prestige.
Biochem/physiol Actions
SERPINA3 (serpin family A member 3) controls the release of leukocyte proteases during an inflammatory response. It has inhibiting nature for several proteases such as mast cell chymase, pancreatic chymotrypsin, human glandular kallikrein 2, kallikrein 3 (prostate-specific antigen), pancreatic cationic elastase and an uncharacterized lung serum protease. It inhibits the function of neutrophil cathepsin G, a pro-inflammatory enzyme and mast cell chymase, which is responsible for the conversion of angiotensin-1 to the active angiotensin-2. SERPINA3 also restricts inflammation, coagulation and extracellular matrix (ECM) remodeling. Overexpression of the protein is responsible for Alzheimer disease and cerebral amyloid encephalopathy. Deficiency of SERPINA3 causes lung and liver disease.
Features and Benefits
Prestige Antibodies® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section.
Every Prestige Antibody is tested in the following ways:
Every Prestige Antibody is tested in the following ways:
- IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues.
- Protein array of 364 human recombinant protein fragments.
Linkage
Corresponding Antigen APREST82413
Physical form
Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide
Legal Information
Prestige Antibodies is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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J P Faber et al.
Journal of hepatology, 18(3), 313-321 (1993-07-01)
Alpha 1-antichymotrypsin (alpha 1-ACT) is a serine proteinase inhibitor (serpin) with cathepsin G, mast cell chymase and chymotrypsin as target enzymes. We present the case of a middle-aged man with low plasma levels of alpha 1-ACT, asthma with progression to
Anita J Horvath et al.
The Journal of biological chemistry, 280(52), 43168-43178 (2005-09-06)
Antichymotrypsin (SERPINA3) is a widely expressed member of the serpin superfamily, required for the regulation of leukocyte proteases released during an inflammatory response and with a permissive role in the development of amyloid encephalopathy. Despite its biological significance, there is
Michael J Bannon et al.
Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology, 39(9), 2191-2199 (2014-03-20)
Chronic drug abuse, craving, and relapse are thought to be linked to long-lasting changes in neural gene expression arising through transcriptional and chromatin-related mechanisms. The key contributions of midbrain dopamine (DA)-synthesizing neurons throughout the addiction process provide a compelling rationale
Sonia T Chelbi et al.
Human molecular genetics, 21(9), 1968-1978 (2012-01-17)
SERPINA3 (Serpin peptidase inhibitor clade A member 3), also known as a1-antichymotrypsin, is a serine protease inhibitor involved in a wide range of biological processes. Recently, it has been shown to be up-regulated in human placental diseases in association with
Mónica Santamaria et al.
Gastroenterology, 144(4), 818-828 (2013-01-09)
α1-Antichymotrypsin (α1-ACT), a member of the serpin family (SERPINA3), is an acute-phase protein secreted by hepatocytes in response to cytokines such as oncostatin M. α1-ACT is a protease inhibitor thought to limit tissue damage produced by excessive inflammation-associated proteolysis. However
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